Tiradentes University, Av. Murilo Dantas, 300, Farolândia, CEP 49030-490, Aracaju/SE, Brazil.
J Photochem Photobiol B. 2011 Oct 5;105(1):51-9. doi: 10.1016/j.jphotobiol.2011.06.009. Epub 2011 Jul 6.
This paper aimed to evaluate the improvement of burn wounds healing by sodium alginate/chitosan-based films and laser therapy. Natural polymers with different biological activities are widely used as film dressings to improve wound healing. Lasers arrays accelerate the healing repair of soft tissue injuries. Burn procedures were performed on the backs of 60 male rats assigned into six groups: untreated (CTR), dressed with cellulose films (CL), dressed with sodium alginate/chitosan-based films (SC), laser-irradiated undressed wounds (LT), laser-irradiated wounds with cellulose (CLLT) and sodium alginate/chitosan-based films (SCLT). Laser therapy was applied for 7 days. Animals of each group were euthanised 8 and 14 days after the burn procedures. The inflammatory reaction was significantly more intense in the CTR group than in the irradiated groups after 8 and 14 days. Laser therapy stimulated myofibroblastic differentiation in 8 days, with or without dressing films. Combined laser therapy and both dressings improved epithelisation, blood vessels formation and collagenization, promoted rapid replacement of type III for type I collagen and favored the better arrangement of the newly formed collagen fibres. The combination of laser therapy and sodium alginate/chitosan-based dressing improves burn healing, apparently by modulating the epithelisation, blood vessels formation and collagenization processes.
本文旨在评估海藻酸钠/壳聚糖基膜和激光治疗对烧伤创面愈合的改善作用。具有不同生物活性的天然聚合物被广泛用作膜敷料，以促进伤口愈合。激光阵列加速软组织损伤的愈合修复。在 60 只雄性大鼠的背部进行烧伤程序，将其分为六组：未处理（对照组）、用纤维素膜（CL）处理、用海藻酸钠/壳聚糖基膜（SC）处理、未经激光处理的激光照射伤口（LT）、激光照射伤口用纤维素（CLLT）和海藻酸钠/壳聚糖基膜（SCLT）处理。激光治疗应用 7 天。烧伤程序后 8 天和 14 天，每组动物均进行安乐死。与照射组相比，对照组在 8 天和 14 天后的炎症反应更强烈。激光治疗在 8 天内刺激肌成纤维细胞分化，有无敷料均可。联合激光治疗和两种敷料均可促进上皮化、血管形成和胶原化，促进 III 型胶原向 I 型胶原的快速替代，并有利于新形成的胶原纤维更好地排列。激光治疗联合海藻酸钠/壳聚糖基敷料可改善烧伤愈合，显然是通过调节上皮化、血管形成和胶原化过程。
