Itaya M, Yamaguchi I, Kobayashi K, Endo T, Tanaka T
Mitsubishi Kasei Institute of Life Sciences, Tokyo.
J Biochem. 1990 Jun;107(6):799-801. doi: 10.1093/oxfordjournals.jbchem.a123128.
The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.
杀稻瘟菌素S抗性基因（bsr）最初是从蜡样芽孢杆菌中分离出来的，本研究在枯草芽孢杆菌中对其进行了研究。研究发现，一个包含完整bsr基因及其5'侧翼区域的617 bp片段，当整合到枯草芽孢杆菌的染色体中时，即使处于单拷贝状态，也能赋予其对杀稻瘟菌素S的抗性。本文报道了bsr基因盒的构建及其作为枯草芽孢杆菌新型选择标记的实际应用。
