Kamakura T, Yoneyama K, Yamaguchi I
Microbial Toxicology Laboratory, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
Mol Gen Genet. 1990 Sep;223(2):332-4. doi: 10.1007/BF00265072.
Blasticidin S (BS), a fungicide of microbial origin, is used for the practical control of rice blast disease. It has broad antimicrobial activity but occasionally exhibits adverse phytotoxic effects on some dicot plants. An inactivating enzyme, BS deaminase, was discovered in the BS resistant strain, Bacillus cereus K55-S1, and the structural gene, bsr, for the enzyme has been cloned. We introduced the bsr gene into tobacco plants using the Ti plasmid vector system and demonstrated that the bsr gene conferred a BS resistant phenotype to the plants. Thus the bsr gene could be useful as a selective marker for plant transformation and provides an example for a new approach to the solution of phytotoxicity problems associated with the use of some types of fungicide.
杀稻瘟菌素S（BS）是一种微生物源杀菌剂，用于实际防治稻瘟病。它具有广泛的抗菌活性，但偶尔会对一些双子叶植物表现出不利的植物毒性作用。在抗BS菌株蜡样芽孢杆菌K55-S1中发现了一种失活酶，即BS脱氨酶，并且已经克隆了该酶的结构基因bsr。我们使用Ti质粒载体系统将bsr基因导入烟草植株，并证明bsr基因赋予了植株抗BS的表型。因此，bsr基因可作为植物转化的选择标记，并为解决与某些类型杀菌剂使用相关的植物毒性问题提供了一种新方法的实例。
