Taiwan was a hyperendemic area for hepatitis A virus (HAV) infection before 1980. The aim of this study was to examine the association between seropositivity of antibodies against HAV (anti-HAV) by a community-based survey. School children from 10 elementary and 3 junior high schools, as well as staff members who worked at the above schools in central Taiwan were selected at random in this study. Anti-HAV was tested in sera of 1,954 healthy schoolchildren (aged 7-15 years old) and 254 teachers by enzyme-linked immunosorbent assay. Schoolchildren had a low prevalence of anti-HAV (2.3%) in contrast to the high seroprevalence in their teachers (52%). The seropositive rates of HAV antibody among the study subjects were increasing with age. No significant differences of anti-HAV seroprevalence among the study subjects were observed when they were stratified by gender, geographical area, household members, and parental education. Whereas, the anti-HAV seroprevalence was significantly higher in schoolchildren who were either aboriginal or living in areas without a supply of drinking tap-water. The seroprevalence of HAV data among the healthy pediatric population would be helpful to evaluate the need for mass vaccination policies.