do Carmo Ferreira Natália, Schuenck Ricardo P, dos Santos Kátia Regina Netto, de Freire Bastos Maria do Carmo, Giambiagi-deMarval Marcia
Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Centro de Ciências da Saúde, Rio de Janeiro, RJ, Brazil.
FEMS Immunol Med Microbiol. 2011 Mar;61(2):147-52. doi: 10.1111/j.1574-695X.2010.00756.x. Epub 2010 Dec 23.
The coagulase-negative staphylococci are known for their ability to acquire resistance genes, which limits the choice of therapeutic options for the treatment of infections caused by these microorganisms. In this study, the diversity of high-level mupirocin resistance plasmids (Mup(R) ) was investigated in four strains of Staphylococcus haemolyticus belonging to different pulsed-field gel electrophoresis (PFGE) types or subtypes, isolated in a Brazilian hospital. These strains harbor the mupA gene in large plasmids. In addition, the presence of IS257 sequences flanking the mupA gene was also shown. Two isolates belonging to two different PFGE types exhibited a similar polymorphism for a fragment of the mupA gene and the closest proximal flanking copies of the IS257, suggesting horizontal transmission of S. haemolyticus mupirocin resistance plasmids in the environment and a role of this species as a reservoir of the mupA gene.
凝固酶阴性葡萄球菌以其获得耐药基因的能力而闻名，这限制了治疗由这些微生物引起的感染的治疗选择。在本研究中，对巴西一家医院分离出的四株属于不同脉冲场凝胶电泳（PFGE）类型或亚型的溶血葡萄球菌菌株中的高水平莫匹罗星耐药质粒（Mup(R)）的多样性进行了研究。这些菌株在大质粒中携带mupA基因。此外，还显示了mupA基因侧翼存在IS257序列。属于两种不同PFGE类型的两个分离株在mupA基因片段和IS257最接近的近端侧翼拷贝上表现出相似的多态性，这表明溶血葡萄球菌莫匹罗星耐药质粒在环境中水平传播，并且该物种作为mupA基因的储存库发挥了作用。
