Cellular and Molecular Research Center, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran.
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
BMC Microbiol. 2024 Jul 25;24(1):276. doi: 10.1186/s12866-024-03430-1.
Hemodialysis patients are at risk of acquiring healthcare-related infections due to using non-sterile water to prepare hemodialysis fluid. Therefore, microbiological control and monitoring of used water are of crucial importance.
In this work, we identified bacterial populations occupying a hemodialysis water distribution system for almost a 6-month period in Ahvaz city, southwest of Iran. A total of 18 samples from three points were collected. We found high colony counts of bacteria on R2A agar. 31 bacteria with different morphological and biochemical characteristics were identified by molecular-genetic methods based on 16 S rRNA gene sequencing. Endotoxin concentrations were measured, using Endosafe Rapid LAL Single-Test Vials.
A diverse bacterial community was identified, containing predominantly Gram-negative bacilli. The most frequently isolated genus was Sphingomonas. Five species including M. fortuitum, M. lentiflavum, M.szulgai, M. barrassiae, and M. gordonae was identified .Despite the presence of Gram-negative bacteria the endotoxin analysis of all samples revealed that their endotoxin values were below the detection limit.
The members of Sphingomonas genus along with Bosea and mycobacteria could be regarded as pioneers in surface colonization and biofilm creation. These bacteria with others like Pelomonas, Bradyrhizobium, staphylococcus, and Microbacterium may represent a potential health risk to patients under hemodialysis treatment.
由于使用非无菌水来制备血液透析液，血液透析患者有感染与医疗保健相关的感染的风险。因此，对使用水进行微生物控制和监测至关重要。
在这项工作中，我们确定了伊朗西南部阿瓦兹市的血液透析水分配系统中近 6 个月内占据的细菌种群。从三个点共采集了 18 个样本。我们发现 R2A 琼脂上的细菌菌落计数很高。通过基于 16S rRNA 基因测序的分子遗传方法鉴定了具有不同形态和生化特征的 31 种细菌。使用 Endosafe Rapid LAL 单测试管测量内毒素浓度。
鉴定出了一个多样化的细菌群落，主要包含革兰氏阴性杆菌。最常分离到的属是鞘氨醇单胞菌。鉴定出了包括 M. fortuitum、M. lentiflavum、M. szulgai、M. barrassiae 和 M. gordonae 在内的 5 个种。尽管存在革兰氏阴性细菌，但所有样本的内毒素分析均表明其内毒素值低于检测限。
鞘氨醇单胞菌属以及 Bosea 和分枝杆菌可被视为表面定植和生物膜形成的先驱。这些与 Pelomonas、Bradyrhizobium、葡萄球菌和 Microbacterium 等细菌可能对接受血液透析治疗的患者构成潜在健康风险。
