Rankl Christian, Zhu Rong, Luengo Gustavo S, Donovan Mark, Baghdadli Nawel, Hinterdorfer Peter
Institute for Biophysics, University of Linz, Linz, Austria.
Exp Dermatol. 2010 Nov;19(11):1014-9. doi: 10.1111/j.1600-0625.2010.01179.x.
Corneodesmosin, a protein known to be present in the stratum corneum (SC), plays an important role in its physical integrity. Here, a specific antibody to corneodesmosin was tethered via a flexible linker to an atomic force microscopy tip, and the interaction forces between this tip and the surface of the SC were successfully measured. Using the recently developed technique of simultaneous topography and recognition imaging, we were able to map the distribution of corneodesmosin on the surface of the SC at the nanoscale.
角质桥粒蛋白是一种已知存在于角质层（SC）中的蛋白质，在维持角质层的物理完整性方面发挥着重要作用。在此，一种针对角质桥粒蛋白的特异性抗体通过柔性连接体连接到原子力显微镜探针上，并成功测量了该探针与角质层表面之间的相互作用力。利用最近开发的同步形貌和识别成像技术，我们能够在纳米尺度上绘制角质桥粒蛋白在角质层表面的分布情况。
