With-Nature Oriental Clinic Group, Republic of Korea.
J Ethnopharmacol. 2011 Mar 24;134(2):281-7. doi: 10.1016/j.jep.2010.12.021. Epub 2010 Dec 21.
Cho-kyung-jong-ok-tang (CKJOT) is a traditional Korean herbal formula specifically used for female infertility including unexplained recurrent pregnancy loss (RPL).
The present study aims to evaluate the effects of CKJOT on mouse natural killer (NK) cells to address the possible immunological basis of protective effects of this herbal medicine on unexplained RPL.
NK cells isolated from spleens of 6-week-old C57BL/6 mice were differentiated into NK0, NK1, and NK2 cells in the presence of various concentrations of CKJOT-extract. Apoptotic cell number, level of intracellular cytokines, and expression of cytokine-related transcription factors were measured.
CKJOT had little effect in improving viability of NK0, NK1, and NK2 cells. However, CKJOT addition during NK cell differentiation suppressed the production of interferon-gamma (IFN-γ), and enhanced that of interleukin-5, in the NK1 and NK2 subsets, respectively. T-bet, a transcription factor associated with IFN-γ expression was down-regulated; while Th2 linked transcription factors (STAT6 and GATA3) were up-regulated especially with 100 μg/mL treatment of CKJOT.
The type 2 shift in NK cell-secreted cytokines induced by CKJOT in mouse NK cells may explain the protective effect associated with its traditional use in unexplained RPL.
朝鮮醫湯（Cho-kyung-jong-ok-tang，CKJOT）是一种传统的韩国草药配方，专门用于治疗女性不孕，包括不明原因的反复妊娠丢失（RPL）。
本研究旨在评估 CKJOT 对小鼠自然杀伤（NK）细胞的影响，以探讨这种草药对不明原因 RPL 保护作用的可能免疫学基础。
从 6 周龄 C57BL/6 小鼠脾脏中分离 NK 细胞，在不同浓度的 CKJOT 提取物存在下将其分化为 NK0、NK1 和 NK2 细胞。测量凋亡细胞数量、细胞内细胞因子水平和细胞因子相关转录因子的表达。
CKJOT 对 NK0、NK1 和 NK2 细胞的活力改善作用不大。然而，在 NK 细胞分化过程中添加 CKJOT 抑制了 NK1 和 NK2 亚群中干扰素-γ（IFN-γ）的产生，分别增强了白细胞介素-5 的产生。与 IFN-γ表达相关的转录因子 T-bet 下调；而 Th2 相关转录因子（STAT6 和 GATA3）上调，特别是用 100μg/mL 的 CKJOT 处理时。
CKJOT 在小鼠 NK 细胞中诱导的 NK 细胞分泌细胞因子的 2 型偏移可能解释了其在不明原因 RPL 中的传统应用相关的保护作用。
