Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität Berlin, Königsweg 65, D-14163 Berlin, Germany.
J Dairy Sci. 2011 Jan;94(1):284-90. doi: 10.3168/jds.2010-3645.
Puerperal uterine infections are often associated with decreased reproductive performance in dairy cows. Routine treatment protocols include the systemic administration of antibiotics. Antibiotic drugs, however, should be administered daily over at least 5 d. The objective of this study was to determine concentrations of ceftiofur derivatives in serum, endometrial tissue, and lochia after subcutaneous administration of ceftiofur crystalline free acid in 6 clinically healthy puerperal dairy cows with normal parturition. Samples were taken immediately before treatment, 2 h after, and then every 24 h over a 7-d period. Concentrations of ceftiofur derivatives were quantified using an HPLC assay. In serum and endometrial tissue, ceftiofur derivatives could be detected above the reported minimum drug concentrations required to inhibit relevant pathogens such as Escherichia coli and Arcanobacterium pyogenes over a 7-d period. Concentrations of desfuroylceftiofuracetamide at 5 d after administration of ceftiofur crystalline free acid were 1.21±0.61 μg/mL in serum, 0.86±0.61 μg/mg in endometrial tissue, and 0.96±1.15 μg/mL in lochia. In lochia, mean concentrations of ceftiofur derivatives also remained above the minimal inhibitory concentration of relevant pathogens, but showed greater variations between cows.
产后子宫感染通常与奶牛的繁殖性能下降有关。常规的治疗方案包括全身使用抗生素。然而，抗生素药物应至少每天给药 5 天。本研究的目的是确定 6 头正常分娩的产后健康奶牛皮下注射头孢噻呋游离酸后，血清、子宫内膜组织和恶露中头孢噻呋衍生物的浓度。在治疗前、治疗后 2 小时以及接下来的 7 天内每 24 小时采集一次样本。使用 HPLC 测定法定量检测头孢噻呋衍生物的浓度。在血清和子宫内膜组织中，头孢噻呋衍生物在 7 天内可检测到高于抑制大肠杆菌和化脓隐秘杆菌等相关病原体所需的最低药物浓度。在给予头孢噻呋游离酸后 5 天，血清中去呋喃甲酰头孢噻呋酰胺的浓度为 1.21±0.61μg/ml，子宫内膜组织中为 0.86±0.61μg/mg，恶露中为 0.96±1.15μg/ml。在恶露中，头孢噻呋衍生物的平均浓度也高于相关病原体的最低抑菌浓度，但在牛之间存在更大的差异。
