Department of Biosciences and Oral Diagnosis, Microbiology and Immunology, São José dos Campos Dental School, Univ Estadual Paulista, Av. Engenheiro Francisco José Longo, 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
Arch Oral Biol. 2011 Jun;56(6):549-55. doi: 10.1016/j.archoralbio.2010.11.018. Epub 2010 Dec 22.
The goal of the study was to measure the prevalence of Candida spp. in the oral cavity of patients with diabetes types 1 and 2 when compared to healthy individuals and to study antifungal resistance profile of the isolates.
There were 162 subjects in the study: diabetes type 1 (n=39); control group 1 (n=50): healthy individuals matched in gender, age, and oral conditions to diabetes type 1 patients; diabetes type 2 (n=37); control group 2 (n=36) who were matched to each patient of the diabetes type 2 group. Stimulated saliva was collected and isolates were identified with phenotypic tests. The presence of C. dubliniensis was determined by multiplex PCR.
There were no statistically significant differences in Candida spp. frequency between the diabetes 1 group and its control (p=0.443) nor between the diabetes 2 group and its control (p=0.429). C. albicans was the most frequently isolated yeast in all groups. In the diabetes groups, C. stellatoidea, C. parapsilosis, C. tropicalis, C. lipolytica, C. glabrata, and C. krusei were also identified. Additionally, in control groups, C. kefyr was also detected. None of the isolates were resistant to amphotericin B and flucytosine. A low percentage of the isolates were resistant to ketoconazole.
No differences were detected in colonization of Candida spp. oral isolates from type 1 and type 2 diabetes when compared to matched controls. The antifungal resistance of Candida spp. isolates for ketoconazole from type 1 diabetes patients was significantly higher than that of its matched control.
本研究旨在比较 1 型和 2 型糖尿病患者与健康个体的口腔念珠菌属（Candida spp.）流行率，并研究分离株的抗真菌耐药谱。
本研究共有 162 名受试者：1 型糖尿病（n=39）；对照组 1（n=50）：性别、年龄和口腔状况与 1 型糖尿病患者相匹配的健康个体；2 型糖尿病（n=37）；对照组 2（n=36），与每位 2 型糖尿病患者相匹配。采集刺激唾液并通过表型试验鉴定分离株。通过多重 PCR 确定是否存在 C. dubliniensis。
1 型糖尿病组与对照组之间（p=0.443）以及 2 型糖尿病组与对照组之间（p=0.429）的念珠菌属（Candida spp.）频率均无统计学差异。在所有组中，最常分离的酵母均为白色念珠菌（C. albicans）。在糖尿病组中，还鉴定出了星状念珠菌（C. stellatoidea）、近平滑念珠菌（C. parapsilosis）、热带念珠菌（C. tropicalis）、解脂念珠菌（C. lipolytica）、光滑念珠菌（C. glabrata）和克柔念珠菌（C. krusei）。此外，在对照组中，还检测到了卡氏酵母（C. kefyr）。所有分离株均对两性霉素 B 和氟胞嘧啶敏感，而对酮康唑的耐药率较低。
与匹配对照组相比，1 型和 2 型糖尿病患者口腔念珠菌属（Candida spp.）分离株的定植情况无差异。1 型糖尿病患者分离的念珠菌属（Candida spp.）对酮康唑的抗真菌耐药性明显高于其匹配对照组。
