Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India.
Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi 110029, India.
J Med Microbiol. 2011 Apr;60(Pt 4):486-491. doi: 10.1099/jmm.0.025080-0. Epub 2010 Dec 23.
This study was designed to test the utility of mRNA-based RT-PCR to detect viable bacilli, indicating active tubercular involvement, and DNA-PCR to detect present or past infection in the diagnosis of active female genital tuberculosis (TB) infection. A total of 200 subjects with complaints of infertility were enrolled in the study. Multiple sampling was done. One hundred and forty-three endometrial aspirate (EA), 94 peritoneal fluid/peritoneal washing (PF/PW) and six cornual biopsy (CB) specimens were collected for diagnosis using microscopy, culture, RT-PCR and DNA-PCR and results were compared with laparoscopic findings. RT-PCR and culture were concordant [positive in four (2.8%) EA specimens] signalling sampling from the site of active infection. Smear microscopy showed a poor detection rate while DNA-PCR showed high positivity. Sixty-one (44.85%) EA specimens, nine (9.57%) PF/PW specimens and two (33.33%) CB specimens were positive by DNA-PCR. Genital TB causing infertility (localized or secondary to TB elsewhere) can be picked up early by DNA-PCR, when it can be completely cured prior to the appearance of florid disease.
本研究旨在测试基于 mRNA 的 RT-PCR 检测活杆菌以指示活动性结核受累的效用，以及 DNA-PCR 检测当前或过去感染在诊断活动性女性生殖器结核（TB）感染中的作用。共有 200 名有不孕症状的患者纳入本研究。进行了多次采样。采集了 143 份子宫内膜抽吸物（EA）、94 份腹膜液/腹膜冲洗液（PF/PW）和 6 份宫角活检（CB）标本，用于显微镜检查、培养、RT-PCR 和 DNA-PCR 诊断，并将结果与腹腔镜检查结果进行比较。RT-PCR 和培养结果一致[在 4 份（2.8%）EA 标本中呈阳性]，表明来自活动性感染部位的采样。涂片显微镜检查显示出较低的检测率，而 DNA-PCR 显示出较高的阳性率。61 份（44.85%）EA 标本、9 份（9.57%）PF/PW 标本和 2 份（33.33%）CB 标本通过 DNA-PCR 呈阳性。通过 DNA-PCR 可以早期发现导致不孕的生殖器 TB（局部或继发于其他部位的 TB），在明显疾病出现之前可以完全治愈。
