新型洞察到紫色色杆菌 968 号中 FK228 生物合成基因簇的遗传组织。
New insights into the genetic organization of the FK228 biosynthetic gene cluster in Chromobacterium violaceum no. 968.
机构信息
Department of Biological Sciences, University of Wisconsin-Milwaukee, P.O. Box 413, Milwaukee, WI 53201, USA.
出版信息
Appl Environ Microbiol. 2011 Feb;77(4):1508-11. doi: 10.1128/AEM.01512-10. Epub 2010 Dec 23.
Abstract
The biosynthetic gene cluster of FK228, an FDA-approved anticancer natural product, was identified and sequenced previously. The genetic organization of this gene cluster has now been delineated through systematic gene deletion and transcriptional analysis. As a result, the gene cluster is redefined to contain 12 genes: depA through depJ, depM, and a newly identified pathway regulatory gene, depR.
摘要
先前已经鉴定并测序了 FDA 批准的抗癌天然产物 FK228 的生物合成基因簇。通过系统的基因缺失和转录分析，现已确定该基因簇的遗传结构。结果表明，该基因簇重新定义为包含 12 个基因：depA 到 depJ、depM 和一个新鉴定的途径调节基因 depR。
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