School of Mathematics, Statistics and Actuarial Science, University of Kent, Canterbury, Kent CT2 7NF, United Kingdom.
J Theor Biol. 2011 Apr 7;274(1):1-11. doi: 10.1016/j.jtbi.2010.12.026. Epub 2010 Dec 22.
A population of [PSI(+)] Saccharomyces cerevisiae cells can be cured of the [PSI(+)] prion by the addition of guanidine hydrochloride (GdnHCl). In this paper we extend existing nucleated polymerisation simulation models to investigate the mechanisms that might underlie curing. Our results are consistent with the belief that prions are dispersed through the cells at division following GdnHCl addition. A key feature of the simulation model is that the probability that a polymer is transmitted from mother to daughter during cell division is dependent upon the length of the polymer. The model is able to reproduce the essential features of data from several different experimental protocols involving addition and removal of GdnHCl.
用盐酸胍（GdnHCl）处理可以使携带[PSI(+)]朊病毒的酿酒酵母细胞群失去[PSI(+)]朊病毒。在本文中，我们扩展了现有的成核聚合模拟模型，以研究可能构成治愈机制的原理。我们的结果与这样一种观点一致，即在 GdnHCl 处理后，朊病毒通过分裂在细胞间扩散。模拟模型的一个关键特征是，在细胞分裂过程中，聚合物从母细胞传递到子细胞的概率取决于聚合物的长度。该模型能够再现涉及添加和去除 GdnHCl 的几种不同实验方案的数据的基本特征。
