Scottish Oceans Institute, University of St Andrews, St Andrews, Fife KY16 8LB, Scotland, UK.
Dev Comp Immunol. 2011 May;35(5):537-44. doi: 10.1016/j.dci.2010.12.012. Epub 2010 Dec 22.
A population of small, mostly undifferentiated, haemocytes were identified and enriched from the circulation of the spider crab, H. araneus, using a two-step density gradient separation procedure. Typically, these cells are spherical, ca. 8-12 μm diameter and have a high nucleus:cytoplasm ratio. Their number in the circulation increases significantly 24 h after a state of haemocytopenia has been created by withdrawal of 2 mL of haemolymph. The rise in the number of these cells at this time is consistent with a left shift phenomenon. A two-step separation procedure was developed to generate enriched populations of these small cells from the haemolymph and in vitro assays revealed that ca. 47% are BrdU-positive in vitro. By contrast BrdU uptake was not observed in the hyaline, semigranular or granular cells. The proliferative ability of the small cells, coupled with their close morphological resemblance to immature haemocytes reported from the haematopoietic tissue of other decapod species, leads us to conclude that these cells are prohaemocytes.
从蜘蛛蟹（H. araneus）的循环系统中，使用两步密度梯度分离程序，鉴定并富集了一小部分未分化的血细胞。这些细胞通常呈球形，直径约为 8-12μm，具有高核质比。在抽取 2 毫升血淋巴导致血细胞减少症后 24 小时，循环系统中这些细胞的数量显著增加。此时，这些细胞数量的增加与左移现象一致。开发了两步分离程序，从血淋巴中产生富含这些小细胞的群体，体外试验表明，约有 47%的小细胞在体外呈 BrdU 阳性。相比之下，在透明细胞、半颗粒细胞或颗粒细胞中未观察到 BrdU 摄取。这些小细胞的增殖能力，以及它们与其他十足目动物造血组织中报道的未成熟血细胞的形态学相似性，使我们得出结论，这些细胞是前血细胞。
