Laboratory of Pathobiology, Department of Zoology, University of Madras, Guindy Campus, Guindy, Chennai 600025, Tamil Nadu, India.
Laboratory of Pathobiology, Department of Zoology, University of Madras, Guindy Campus, Guindy, Chennai 600025, Tamil Nadu, India.
J Invertebr Pathol. 2017 Sep;148:129-137. doi: 10.1016/j.jip.2017.06.011. Epub 2017 Jun 28.
The mole crab, Emerita emeritus, collected from the sandy shores of a Chennai beach, was investigated for cellular immune responses based on the morphology and defensive reactions of the circulating haemocytes. Three haemocyte morphotypes were identified using light and electron microscopy, and separated in a discontinuous percoll gradient. A phagocytosis study using human B erythrocyte as a target cell under phase-contrast optics showed that granular and semi-granular haemocytes were phagocytic, and this response was enhanced by using serum (opsonin)-coated human B erythrocyte in unfractionated and fractionated haemocytes. Observation of TEM image of phagocytosis revealed that the initial recognition and binding of the target cell was restricted to granular and semigranular haemocytes, which were lacking with hyaline cells. However, the encapsulation of DEAE Sepharose CL 6B beads, either untreated or coated with serum (opsonin), was restricted to hyaline cells. This suggests the occurrence of two cell lines in haemocytes, based on the differences observed in the response of haemocytes to bind target cells for phagocytosis or encapsulation. The present study also differentiated the activation of PO in the plasma, serum, and haemocyte lysate supernatant (HLS).
从钦奈海滩的沙质海岸采集的豆蟹（Emerita emeritus），基于循环血细胞的形态和防御反应，对其进行了细胞免疫反应的研究。使用光镜和电子显微镜鉴定了三种血细胞形态，并在不连续的聚蔗糖梯度中分离。在相差光学显微镜下用人 B 红细胞作为靶细胞进行吞噬作用研究表明，颗粒状和半颗粒状血细胞具有吞噬作用，而使用包被血清（调理素）的人 B 红细胞可增强未分级和分级血细胞的吞噬作用。吞噬作用的 TEM 图像观察表明，靶细胞的初始识别和结合仅限于颗粒状和半颗粒状血细胞，而透明细胞则缺乏。然而，DEAE 琼脂糖 CL 6B 珠的包被，无论是未经处理还是用血清（调理素）包被，都仅限于透明细胞。这表明血细胞中存在两种细胞系，这是基于血细胞对结合靶细胞进行吞噬或包被的反应差异观察得出的。本研究还区分了血浆、血清和血细胞裂解上清液（HLS）中 PO 的激活。
