Department of Cardiology, Hillerod Hospital, Hillerod, Denmark.
Clin Biochem. 2011 Mar;44(4):312-8. doi: 10.1016/j.clinbiochem.2010.12.010. Epub 2010 Dec 23.
To investigate if pregnancy associated plasma protein-A (PAPP-A) was present in the vulnerable plaque, and if not, to find alternative hypothesis for the release of PAPP-A.
Vulnerable plaques and control tissues were examined by immunohistochemistry. Volunteers and patients with non-atherosclerotic disease were examined for release of PAPP-A during ischemia and medical treatment. Non-atherosclerotic tissue samples were examined after incubation with heparins.
We were not able to detect PAPP-A in vulnerable plaques. Patients and volunteers experiencing ischemic events without atherosclerotic lesions only had elevated PAPP-A when treated with heparin. When tissue from normal artery wall was incubated with heparin, PAPP-A was eluted. This was not the case for non-arterial tissue samples.
Elevation of PAPP-A in patients with acute coronary syndromes seems to be caused by heparin induced release of PAPP-A from the arterial wall and not due to excretion from vulnerable plaques.
研究妊娠相关血浆蛋白 A（PAPP-A）是否存在于易损斑块中，如果不存在，则寻找 PAPP-A 释放的替代假说。
通过免疫组织化学方法检查易损斑块和对照组织。检查非动脉粥样硬化性疾病的志愿者和患者在缺血和药物治疗期间 PAPP-A 的释放情况。用肝素孵育后检查非动脉粥样硬化组织样本。
我们未能在易损斑块中检测到 PAPP-A。经历无动脉粥样硬化病变的缺血事件的患者和志愿者仅在用肝素治疗时 PAPP-A 升高。当正常动脉壁组织用肝素孵育时，PAPP-A 被洗脱。非动脉组织样本则不是这种情况。
急性冠状动脉综合征患者 PAPP-A 的升高似乎是由肝素诱导的动脉壁 PAPP-A 释放引起的，而不是来自易损斑块的排泄。
