Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR 97331, USA.
J Virol Methods. 2011 Mar;172(1-2):81-4. doi: 10.1016/j.jviromet.2010.12.012. Epub 2010 Dec 23.
Koi Herpes Virus (KHV) has been classified recently as a member of the Alloherpesviridae within the Herpesvirales order (Waltzek et al., 2005). Although one of the unique features of Herpesviridae, the sister family of Herpesvirales, is latent infection, it has not been demonstrated consistently that KHV of Alloherpesviridae can cause latent infection and be reactivated from latency. To investigate if KHV genomic DNA is present in koi exposed to KHV infection, 10 healthy fish were investigated from a koi population with a history of a KHV outbreak. No gross lesions or microscopic changes were observed at necropsy or by histological examination. No infectious virus was isolated from either the blood plasma or tissues. However, KHV DNA was detected in the white blood cells of nine of the ten fish by real-time PCR and PCR-Southern blot. KHV DNA was also detected in the brain, eye, spleen, gills hematopoietic kidney, trunk kidney, and intestine of nine of the ten fish by PCR-Southern blot. Interestingly, KHV DNA was also detected in the intestinal contents from seven of ten koi. Portions of major capsid gene DNA, amplified from two of the ten koi WBCs, were found to be identical to KHV-U. This study demonstrated that KHV genomic DNA can be detected in normal koi exposed previously to KHV and suggests that KHV becomes latent in fish.
锦鲤疱疹病毒（KHV）最近被归类为疱疹病毒目（Herpesvirales）中的 Alloherpesviridae 成员（Waltzek 等人，2005 年）。虽然疱疹病毒科（Herpesviridae）是疱疹病毒目（Herpesvirales）的姐妹家族，其独特特征之一是潜伏感染，但尚未一致证明 Alloherpesviridae 的 KHV 可以引起潜伏感染并从潜伏中重新激活。为了研究锦鲤是否存在暴露于 KHV 感染的锦鲤的 KHV 基因组 DNA，从有 KHV 暴发史的锦鲤种群中检查了 10 条健康鱼。尸检或组织学检查均未观察到明显病变或显微镜下变化。无论是从血浆还是组织中均未分离出传染性病毒。然而，通过实时 PCR 和 PCR- Southern blot 在十只鱼中的九只的白细胞中检测到了 KHV DNA。通过 PCR- Southern blot 在九只鱼中的九只的大脑、眼睛、脾脏、鳃造血肾、躯干肾和肠中也检测到了 KHV DNA。有趣的是，在十只锦鲤中的七只的肠内容物中也检测到了 KHV DNA。从十只锦鲤中的两只 WBC 扩增的主要衣壳基因 DNA 的部分被发现与 KHV-U 相同。这项研究表明，先前暴露于 KHV 的正常锦鲤中可以检测到 KHV 基因组 DNA，并表明 KHV 在鱼类中潜伏。
