Instituto de Biotecnología, CICVyA-INTA, N. Repetto y De Reseros, 1686 Hurlingham, Buenos Aires, Argentina.
Tuberculosis (Edinb). 2011 Jan;91(1):57-63. doi: 10.1016/j.tube.2010.11.007. Epub 2010 Dec 24.
The identification of bovine tuberculosis (bTB) biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and to improve the disease diagnosis and prognosis. The aim of this study was to understand the changing profile of the immune responses during the course of infection and to identify biomarkers associated with pathology. Here we describe the immune response developed in experimentally infected cattle with field Mycobacterium bovis strains. Blood samples were taken from each animal at different time points after M. bovis intratracheal infection and lymphocyte subset activation and cytokine mRNA expression were determined from peripheral blood mononuclear cells in response to purified protein derivative (PPDB). We found that CD4 and CD8 activation during the early stages of infection, together with IL-17 gene expression, were positively associated with pathology. The results of this study provide evidences of the role of IL-17 in the immunopathology of tuberculosis and support the use of IL-17 as a potential biomarker with predictive value of prognosis in bTB.
鉴定牛结核病（bTB）在疾病特定阶段的生物标志物，有助于更好地了解与结核病相关的免疫病理学，并改善疾病的诊断和预后。本研究的目的是了解感染过程中免疫反应的变化特征，并鉴定与病理学相关的生物标志物。在此，我们描述了用田间分枝杆菌菌株感染的实验牛中产生的免疫反应。在经气管内感染 M. bovis 后，在不同时间点从每只动物采集血液样本，并从外周血单核细胞中测定对纯化蛋白衍生物（PPDB）的淋巴细胞亚群激活和细胞因子 mRNA 表达。我们发现，感染早期 CD4 和 CD8 的激活以及 IL-17 基因表达与病理学呈正相关。本研究结果提供了 IL-17 在结核病免疫病理学中的作用证据，并支持将 IL-17 用作 bTB 具有预后预测价值的潜在生物标志物。
