Lookingland K J, Gunnet J W, Toney T W, Moore K E
Department of Pharmacology and Toxicology, Michigan State University, East Lansing.
Neuroendocrinology. 1990 Jul;52(1):99-105. doi: 10.1159/000125546.
The purpose of the present study was to characterize the acute inhibitory effects of restraint stress on the activity of tuberoinfundibular dopamine (DA) neurons as estimated by measuring concentrations of 3,4-dihydroxyphenylacetic acid (DOPAC) in the median eminence. The time course of the effects of two types of physical restraint (immobilization in the supine position or confinement in an acrylic cylindrical tube) was determined in unanesthetized and diethylether (ether)-exposed female and male rats. The combination of brief (2 min) exposure to ether followed by 10 and 20 min of supine restraint increased concentrations of prolactin in plasma and decreased DOPAC concentrations in median eminence of both female and male rats. Thirty minutes of supine restraint decreased DOPAC concentrations in the median eminence of female rats that were not exposed to ether, and brief exposure to ether enhanced this effect. By contrast, 30 min of supine restraint failed to alter DOPAC concentrations in the median eminence in either unanesthetized or ether-exposed male rats. Tube restraint in the absence of ether failed to alter DOPAC concentrations in the median eminence of either female or male rats; but in female rats preexposed to ether, 30 min of tube restraint decreased DOPAC concentrations in the median eminence. On the other hand, in the absence of physical restraint, 2 min ether exposure caused a transient increase in prolactin secretion and a concurrent decrease of DOPAC concentrations in median eminence of both female and male rats.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究的目的是通过测量正中隆起中3,4-二羟基苯乙酸（DOPAC）的浓度来表征束缚应激对结节漏斗多巴胺（DA）神经元活性的急性抑制作用。在未麻醉和暴露于二乙醚（乙醚）的雌性和雄性大鼠中，确定了两种身体束缚方式（仰卧位固定或限制在丙烯酸圆柱管中）的作用时间进程。短暂（2分钟）暴露于乙醚后，再进行10分钟和20分钟的仰卧位束缚，会增加雌性和雄性大鼠血浆中催乳素的浓度，并降低正中隆起中DOPAC的浓度。30分钟的仰卧位束缚会降低未暴露于乙醚的雌性大鼠正中隆起中DOPAC的浓度，短暂暴露于乙醚会增强这种作用。相比之下，30分钟的仰卧位束缚未能改变未麻醉或暴露于乙醚的雄性大鼠正中隆起中DOPAC的浓度。在没有乙醚的情况下，管束缚未能改变雌性或雄性大鼠正中隆起中DOPAC的浓度；但在预先暴露于乙醚的雌性大鼠中，30分钟的管束缚会降低正中隆起中DOPAC的浓度。另一方面，在没有身体束缚的情况下，2分钟的乙醚暴露会导致雌性和雄性大鼠催乳素分泌短暂增加，同时正中隆起中DOPAC的浓度降低。（摘要截短至250字）
