State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2011 Feb;78(2):700-5. doi: 10.1016/j.saa.2010.11.052. Epub 2010 Dec 7.
Nonionic fluorosurfactant (FSN)-capped gold nanoparticles (GNPs) remain excellently stable at a wider pH range and high ionic strength, which is useful to investigate some CL systems involved in high salt and a strict pH range. In this study, we utilized FSN-capped GNPs of different sizes to distinguish the emitting species from H2O2-Co2+-NaOH and H2O2-Co2+-NaHCO3 systems. When the pH of FSN-capped gold colloidal solution was adjusted to 10.2 by dropwise addition of 0.05 M NaOH, the CL intensity of H2O2-Co2+-NaHCO3 system was enhanced 6-fold or 60-fold respectively in the presence of FSN-capped 14 nm or 69 nm GNPs with comparison to H2O2-Co2+-NaOH. The variation of CL spectra and UV-vis spectra, as well as the quenching effect of reactive oxygen species scavengers were studied in detail to understand the CL enhancement mechanisms of FSN-capped GNPs on the two systems. For H2O2-Co2+-NaOH system, the gold(I) complexes intermediate and singlet oxygen dimol species were proposed as the emitting species. The excited states of the carbon dioxide dimers and singlet oxygen dimol species were considered responsible for the light emission of H2O2-Co2+-NaHCO3 system. To our knowledge, this work is the first time to study the two CL systems simultaneously using nanoparticles.
非离子氟表面活性剂（FSN）包覆的金纳米粒子（GNPs）在更宽的 pH 范围和高离子强度下仍保持极好的稳定性，这对于研究涉及高盐和严格 pH 范围的某些 CL 体系非常有用。在本研究中，我们利用不同尺寸的 FSN 包覆的 GNPs 来区分 H2O2-Co2+-NaOH 和 H2O2-Co2+-NaHCO3 体系中的发光物质。当通过逐滴加入 0.05 M NaOH 将 FSN 包覆的金胶体溶液的 pH 值调节至 10.2 时，与 H2O2-Co2+-NaOH 相比，在存在 FSN 包覆的 14nm 或 69nm GNPs 的情况下，H2O2-Co2+-NaHCO3 体系的 CL 强度分别增强了 6 倍或 60 倍。详细研究了 CL 光谱和紫外-可见光谱的变化以及活性氧物种猝灭剂的猝灭效应，以了解 FSN 包覆的 GNPs 对这两个体系的 CL 增强机制。对于 H2O2-Co2+-NaOH 体系，提出了金（I）配合物中间体和单线态氧双分子物种作为发光物质。二氧化碳二聚体和单线态氧双分子物种的激发态被认为是 H2O2-Co2+-NaHCO3 体系发光的原因。据我们所知，这是首次使用纳米粒子同时研究这两个 CL 体系。
