Department of Oncology, Clinical Sciences, Lund University, Sweden.
J Proteome Res. 2011 Mar 4;10(3):1416-9. doi: 10.1021/pr1011476. Epub 2011 Feb 3.
In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
在 Western blot 中，通常会进行管家蛋白的免疫检测，以检测电泳加载中的差异。本工作描述了一种更快、更简单的蛋白质染色方法，与普通的封闭条件兼容。此外，与普通染色方法相比，该方法在免疫检测后具有更好的线性度。免疫印迹和染色后，可以使用肽质量指纹图谱进一步鉴定蛋白质条带。
