Steinmassl M, Wolf G
Institut für Med. Mikrobiologie, Infektions-und Seuchenlehre, Tierärztlichen Fakultät der Ludwig-Maximilians-Universität.
Zentralbl Veterinarmed B. 1990 Jul;37(5):321-31.
A new model was established to investigate the release of cytokines by porcine peripheral mononuclear leukocytes (MNL). Thereafter, this model is suitable to test paramunity inducers (biological response modifiers) and might replace animal models. The paramunity inducers Pind AVI and Pind ORF as well as other preparations of pox- and paramyxoviruses were employed. Production of both Interleukin 2 (IL2) and Interferon alpha (IFN alpha) was evaluated. Virus containing fluids induced an increase in IL2 activity (CTLL-2 proliferation assay) and IFN alpha (MDBK-cell line, VSV-virus) was noted. The release of IFN alpha and IL2 is caused by viral components. Pind AVI and Pind ORF induce production of IFN alpha in porcine MNL to a similar amount as ND-virus does. All virus preparations caused an increase in IL2 activity in supernatants of resting as well as mitogen-activated MNL without prior sensitization of the cells. The highest IL2-activity was measured after co-stimulation of the MNL with Concanavalin A and ND-virus.
建立了一种新模型，用于研究猪外周血单个核白细胞（MNL）释放细胞因子的情况。此后，该模型适用于测试免疫增强诱导剂（生物反应调节剂），并可能取代动物模型。使用了免疫增强诱导剂Pind AVI和Pind ORF以及痘病毒和副粘病毒的其他制剂。评估了白细胞介素2（IL2）和α干扰素（IFNα）的产生。含病毒的液体可诱导IL2活性增加（CTLL-2增殖试验），并观察到IFNα（MDBK细胞系，VSV病毒）增加。IFNα和IL2的释放是由病毒成分引起的。Pind AVI和Pind ORF诱导猪MNL产生IFNα的量与新城疫病毒（ND病毒）相似。所有病毒制剂均可使静息和丝裂原激活的MNL上清液中的IL2活性增加，而无需事先使细胞致敏。在用伴刀豆球蛋白A和ND病毒共同刺激MNL后，测得的IL2活性最高。
