Biological and Soil Faculty, St. Petersburg State University, St. Petersburg, Russia.
Respir Physiol Neurobiol. 2011 Mar 15;175(3):331-5. doi: 10.1016/j.resp.2010.12.012. Epub 2010 Dec 25.
The permeability of pleural mesothelium helps to control the volume and composition of the liquid lubricating pleural surfaces. Information on pleural barrier function in health and disease, however, is scarce. Tissue specimens of human pleura were mounted in Ussing chambers for measurement of transmesothelial resistance. Expression of tight junction (TJ) proteins was studied by Western blots and immune fluorescence confocal microscopy. Both visceral and parietal pleura showed barrier properties represented by transmesothelial resistance. Occludin, claudin-1, -3, -5, and -7, were detected in visceral pleura. In parietal pleura, the same TJ proteins were detected, except claudin-7. In tissues from patients with pleural inflammation these tightening claudins were decreased and in visceral pleura claudin-2, a paracellular channel former, became apparent. We report that barrier function in human pleura coincides with expression of claudins known to be key determinants of epithelial barrier properties. In inflamed tissue, claudin expression indicates a reduced barrier function.
胸膜间皮的通透性有助于控制胸膜表面液体的润滑量和组成。然而，关于胸膜屏障功能在健康和疾病中的信息却很少。将人体胸膜组织标本装入 Ussing 室，以测量跨胸膜电阻。通过 Western blot 和免疫荧光共聚焦显微镜研究紧密连接 (TJ) 蛋白的表达。内脏胸膜和壁胸膜均表现出以跨胸膜电阻为特征的屏障特性。在脏层胸膜中检测到闭合蛋白、claudin-1、-3、-5 和 -7。在壁胸膜中，除了 claudin-7 外，还检测到相同的 TJ 蛋白。在有胸膜炎症的组织中，这些收紧的紧密连接蛋白减少，而在脏层胸膜中，一种旁细胞通道形成蛋白，claudin-2 变得明显。我们报告说，人胸膜的屏障功能与紧密连接蛋白的表达一致，这些紧密连接蛋白是上皮屏障特性的关键决定因素。在炎症组织中，紧密连接蛋白的表达表明屏障功能降低。
