Tazawa H
Dept. of Ophthalmol., Niigata Univ. School of Med., Japan.
Nippon Ganka Gakkai Zasshi. 1990 Mar;94(3):269-76.
Adherence of bacteria to the corneal epithelium is the first step in the pathogenesis of corneal infection. Keratitis caused by Pseudomonas aeruginosa usually occurs among the contact lens wearers. Adherence of Pseudomonas aeruginosa to rabbit corneal epithelium, damaged by one week of hard contact lens wear, was examined histologically. The cornea was excised for scanning electron microscopy at 5, 15, 30 and 60 minutes after inoculation of Pseudomonas aeruginosa (0.2 ml, 10(8)CFU/ml). Pseudomonas aeruginosa did not adhere to the intact corneal epithelium, but traumatized cornea provided a site for adherence. In rabbits in which the eyelid was opened by lid retractors, large numbers of organisms were observed adhering to the injured cornea mediated by ocular surface mucin. Thirty minutes after inoculation, the adherent bacteria began to penetrate the epithelial cells and surface mucin by the formation of pockets surrounding the organism.
细菌黏附于角膜上皮是角膜感染发病机制的第一步。铜绿假单胞菌引起的角膜炎通常发生在隐形眼镜佩戴者中。通过组织学方法检查了铜绿假单胞菌对因佩戴一周硬性隐形眼镜而受损的兔角膜上皮的黏附情况。接种铜绿假单胞菌（0.2 ml，10⁸CFU/ml）后5、15、30和60分钟，切除角膜用于扫描电子显微镜检查。铜绿假单胞菌不黏附完整的角膜上皮，但受损的角膜提供了黏附位点。在使用眼睑牵开器打开眼睑的兔子中，观察到大量细菌通过眼表黏蛋白介导黏附于受伤的角膜。接种30分钟后，黏附的细菌开始通过围绕细菌形成小窝穿透上皮细胞和表面黏蛋白。
