Stern G A, Lubniewski A, Allen C
Arch Ophthalmol. 1985 Aug;103(8):1221-5. doi: 10.1001/archopht.1985.01050080133033.
The interaction between Pseudomonas aeruginosa and the rabbit corneal epithelium during the first hour after inoculation was studied using scanning and transmission electron microscopy. Fifteen minutes after inoculation, adherent bacteria were found on damaged or exposed basal epithelial cells at the edge of an epithelial defect, and this adherence was the result of an interaction between the bacterial and epithelial cell membranes. Thirty minutes after inoculation, the adherent bacteria began to penetrate the epithelial cells by the formation of "pockets" surrounding the organisms, and after an additional 15 minutes the bacteria further penetrated the cells as the pockets began to fill in with cellular material. One hour after inoculation, only rare bacteria were seen on the cell surfaces or within the margins of the epithelial defect. Transmission electron microscopy, however, revealed many intracellular bacteria or bacteria that had migrated between the basal epithelium and corneal stroma. Therefore, it appears that the initiating events in Pseudomonas corneal ulceration involve adherence to the damaged or exposed basal epithelial cells by an interaction between the bacterial and cellular membranes, after which the organisms are engulfed by the epithelial cell and reach the corneal stroma by a process of transcellular migration.
利用扫描电子显微镜和透射电子显微镜研究了接种后第一小时铜绿假单胞菌与兔角膜上皮之间的相互作用。接种15分钟后，在上皮缺损边缘受损或暴露的基底上皮细胞上发现了黏附的细菌，这种黏附是细菌细胞膜与上皮细胞膜相互作用的结果。接种30分钟后，黏附的细菌开始通过围绕菌体形成“小窝”来穿透上皮细胞，再过15分钟，随着小窝开始被细胞物质填满，细菌进一步穿透细胞。接种1小时后，在细胞表面或上皮缺损边缘仅可见少数细菌。然而，透射电子显微镜显示有许多细胞内细菌或已在基底上皮和角膜基质之间迁移的细菌。因此，看来铜绿假单胞菌角膜溃疡的起始事件包括通过细菌细胞膜与上皮细胞膜的相互作用黏附于受损或暴露的基底上皮细胞，此后菌体被上皮细胞吞噬，并通过跨细胞迁移过程到达角膜基质。
