不同组织来源的间充质干细胞表达癌/睾丸抗原。
Cancer/Testis antigen expression on mesenchymal stem cells isolated from different tissues.
机构信息
Fundação Hemocentro de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
出版信息
Anticancer Res. 2010 Dec;30(12):5023-7.
BACKGROUND/AIMS: The expression of cancer/testis antigens (CTAs) on additional normal tissues or stem cells may restrict their use as cancer targets. The objective of the present study was to evaluate the mRNA levels of some CTAs in a variety of tissues.
MATERIALS AND METHODS
mRNA of pericytes, fibroblasts and mesenchymal stem cells (MSCs) derived from adult and fetal tissues, human umbilical vein endothelial cells, MSC-derived adipocytes, selected normal tissues and control cancer cell lines (CLs) were extracted and quantitative polymerase chain reaction was performed for MAGED1, PRAME, CTAG1B, MAGEA3 and MAGEA4.
RESULTS
MAGED1 was expressed in all normal tissues and cells evaluated. CTAG1B was expressed at levels comparable to control CLs on MSCs derived from arterial, fetal skin, adipose tissue and saphenous vein, heart, brain and skin tissues. MAGEA4 was detected only in fibroblasts and differentiated adipocytes from MSCs, at levels comparable to the control CLs.
CONCLUSION
The potential use of CTAs in immunotherapy should take into account the potential off-target effects on MSCs.
背景/目的：癌症/睾丸抗原（CTAs）在其他正常组织或干细胞上的表达可能会限制它们作为癌症靶点的应用。本研究的目的是评估多种组织中某些 CTA 的 mRNA 水平。
材料和方法
提取来自成人和胎儿组织的周细胞、成纤维细胞和间充质干细胞（MSCs）、人脐静脉内皮细胞、MSC 衍生的脂肪细胞、选定的正常组织和对照癌细胞系（CLs）的 mRNA，并进行 MAGED1、PRAME、CTAG1B、MAGEA3 和 MAGEA4 的定量聚合酶链反应。
结果
MAGED1 在评估的所有正常组织和细胞中均有表达。CTAG1B 在动脉、胎儿皮肤、脂肪组织和大隐静脉、心脏、大脑和皮肤组织来源的 MSC 上与对照 CLs 相当的水平表达。MAGEA4 仅在成纤维细胞和 MSC 分化的脂肪细胞中检测到，与对照 CLs 相当的水平。
结论
在免疫治疗中使用 CTA 时，应考虑到对 MSC 的潜在脱靶效应。
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