用于估计人布鲁氏菌病诊断中IgG和IgM抗布鲁氏菌抗体的细胞免疫酶法的比较研究。

Comparative study of cell-immunoenzymatic methods for the estimation of IgG and IgM anti-Brucella antibodies in the diagnosis of human brucellosis.

作者信息

Mongini C, Fernández T, Turovetzky A, Hajos S E

机构信息

Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.

出版信息

J Appl Bacteriol. 1990 Jul;69(1):86-91. doi: 10.1111/j.1365-2672.1990.tb02915.x.

DOI:10.1111/j.1365-2672.1990.tb02915.x

PMID:2118899

Abstract

Two methods which employ whole cells are described and compared for the detection of human IgG and IgM anti-brucella antibodies. Dot ELISA and ELISA were shown to be suitable for a screening diagnosis of human brucellosis. Titres of antibodies obtained by dot ELISA showed 100% coincidence for IgG and 97% for IgM, compared with agglutination and complement fixation tests; when ELISA was used 11% positive sera were not detected. The comparison of these two methods with the conventional serological test kit indicated that both dot ELISA and ELISA were sensitive, reproducible and specific for the quantification of IgG and IgM antibrucella antibodies.

摘要

描述并比较了两种使用全细胞检测人抗布鲁氏菌 IgG 和 IgM 抗体的方法。斑点酶联免疫吸附测定法（Dot ELISA）和酶联免疫吸附测定法（ELISA）被证明适用于人类布鲁氏菌病的筛查诊断。与凝集试验和补体结合试验相比，通过斑点酶联免疫吸附测定法获得的抗体滴度显示 IgG 为 100% 相符，IgM 为 97% 相符；使用酶联免疫吸附测定法时，11% 的阳性血清未被检测到。这两种方法与传统血清学检测试剂盒的比较表明，斑点酶联免疫吸附测定法和酶联免疫吸附测定法对于定量抗布鲁氏菌 IgG 和 IgM 抗体均具有敏感性、可重复性和特异性。