采用斑点酶联免疫吸附测定法检测人体中抗布鲁氏菌的IgM和IgG抗体。

Detection of IgM and IgG antibodies against Brucella by dot-ELISA in humans.

作者信息

Barbuddhe S B, Yadava V K, Singh D K

机构信息

Division of Veterinary Public Health, Indian Veterinary Research Institute.

出版信息

J Commun Dis. 1994 Mar;26(1):1-5.

PMID:7963376

Abstract

Dot-enzyme linked immunosorbent assay (dot-ELISA) with autoclaved extract of B. abortus S99 was used for the detection of Brucella antibodies in human sera. Results were compared with those of STAT, RBPT and CFT. Out of 80 sera 8(10 per cent), 15(18.7 per cent), 20(25 per cent) and 13(16.25 per cent) were found positive by RBPT, STAT, IgM dot-ELISA and IgG dot-ELISA respectively. Out of 74 sera samples tested with CFT 9(12.16 per cent) proved positive. The relative sensitivity and relative specificity in comparison with CFT was found to be 33.33 per cent, 96.92 percent for RBPT, 33.33 per cent and 84.61 per cent for STAT and 88.88 per cent and 76.92 per cent for dot-ELISA, respectively. The dot-ELISA was found to be a more sensitive, economical and a rapid test for screening of human brucellosis under field conditions.

摘要

采用流产布鲁氏菌S99高压灭菌提取物的斑点酶联免疫吸附测定（dot-ELISA）检测人血清中的布鲁氏菌抗体。将结果与虎红平板凝集试验（STAT）、试管凝集试验（RBPT）和补体结合试验（CFT）的结果进行比较。在80份血清中，RBPT、STAT、IgM斑点酶联免疫吸附测定和IgG斑点酶联免疫吸附测定分别检测出8份（10%）、15份（18.7%）、20份（25%）和13份（16.25%）阳性。在用补体结合试验检测的74份血清样本中，9份（12.16%）结果呈阳性。与补体结合试验相比，RBPT的相对敏感性和相对特异性分别为33.33%和96.92%，STAT为33.33%和84.61%，斑点酶联免疫吸附测定为88.88%和76.92%。结果表明，在现场条件下，斑点酶联免疫吸附测定是一种更敏感、经济且快速的人布鲁氏菌病筛查检测方法。