Unit of Immunology and Microbiology Environmental and Carcinogenesis, Faculty of Sciences of Bizerte, 7021 Zarzouna, University of 7-November at Carthage, Tunisia.
J Exp Clin Cancer Res. 2010 Dec 28;29(1):171. doi: 10.1186/1756-9966-29-171.
The present study was undertaken to relate the co-expression of prostate-associated antigens, PSMA and PSA, with the degree of vascularization in normal and pathologic (hyperplasia and cancer) prostate tissues to elucidate their possible role in tumor progression.
The study was carried out in 6 normal, 44 benign prostatic hyperplastic and 39 cancerous human prostates. Immunohistochemical analysis were performed using the monoclonal antibody CD34 to determine the angiogenic activity, and the monoclonal antibodies 3E6 and ER-PR8 to assess PSMA and PSA expression, respectively.
In our study we found that in normal prostate tissue, PSMA and PSA were equally expressed (3.7 ± 0.18 and 3.07 ± 0.11). A significant difference in their expression was see in hyperplastic and neoplastic prostates tissues (16.14 ± 0.17 and 30.72 ± 0.85, respectively) for PSMA and (34.39 ± 0.53 and 17.85 ± 1.21, respectively) for PSA. Study of prostate tumor profiles showed that the profile (PSA+, PSMA-) expression levels decreased between normal prostate, benign prostatic tissue and primary prostate cancer. In the other hand, the profile (PSA-, PSMA+) expression levels increased from normal to prostate tumor tissues. PSMA overexpression was associated with high intratumoral angiogenesis activity. By contrast, high PSA expression was associated with low angiogenesis activity.
These data suggest that these markers are regulated differentially and the difference in their expression showed a correlation with malignant transformation. With regard to the duality PSMA-PSA, this implies the significance of their investigation together in normal and pathologic prostate tissues.
本研究旨在探讨前列腺相关抗原 PSMA 和 PSA 的共表达与正常和病变（增生和癌）前列腺组织中血管生成程度的关系，以阐明它们在肿瘤进展中的可能作用。
本研究在 6 例正常、44 例良性前列腺增生和 39 例前列腺癌患者中进行。使用单克隆抗体 CD34 进行免疫组织化学分析，以确定血管生成活性，并用单克隆抗体 3E6 和 ER-PR8 分别评估 PSMA 和 PSA 的表达。
在我们的研究中，我们发现正常前列腺组织中 PSMA 和 PSA 的表达水平相等（3.7 ± 0.18 和 3.07 ± 0.11）。在增生和肿瘤前列腺组织中，PSMA 的表达差异显著（分别为 16.14 ± 0.17 和 30.72 ± 0.85），PSA 的表达差异也显著（分别为 34.39 ± 0.53 和 17.85 ± 1.21）。对前列腺肿瘤谱的研究表明，在正常前列腺、良性前列腺组织和原发性前列腺癌中，PSA+，PSMA-表达水平降低。另一方面，PSA-，PSMA+表达水平从正常前列腺组织升高到前列腺肿瘤组织。PSMA 过表达与肿瘤内高血管生成活性相关。相比之下，高 PSA 表达与低血管生成活性相关。
这些数据表明，这些标志物的表达受到不同的调控，其表达的差异与恶性转化相关。关于 PSMA-PSA 的双重性，这意味着在正常和病变前列腺组织中同时研究它们具有重要意义。
