Lee Min-Jung, Han Kook-Jin, Kwon Hyuk-Jae, Jung Han-Sung, Cho Sung-Won
Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, Korea.
Anat Cell Biol. 2010 Jun;43(2):125-31. doi: 10.5115/acb.2010.43.2.125. Epub 2010 Jun 30.
Nitric oxide is one of many proinflammatory mediators that are involved in temporomandibular joint (TMJ) inflammatory disorder and is synthesized by inducible nitric oxide synthase (iNOS). iNOS is transcriptionally regulated by nuclear factor-κB (NF-κB) in cases of inflammation, proliferation, and apoptosis. It has also been reported that nitric oxide is positively regulated by carrageenan and negatively regulated by hyaluronan in the knee joint. The aim of this study was to histologically evaluate how inflammation and cell proliferation of the synovial membrane are affected by the exogenous administration of carrageenan and hyaluronan in the rat TMJ by investigating iNOS, NF-κB, and anti proliferating cell nuclear antigen (PCNA) immunoreactivity. As results, immunoreactive cells to iNOS, NF-κB, and PCNA were normally localized only in the synovial membrane of wild type TMJs. The numbers of immunoreactive cells were extensively larger in the carrageenan-injected synovial membranes exhibiting excessive folding, and smaller in the hyaluronan-injected synovial membranes showing a few folds. These results indicate that a carrageenan injection induced inflammation and cell proliferation especially in the synovial membrane and that hyaluronan relieved the inflammation by decreasing inflammatory molecules in the synovial membrane.
一氧化氮是参与颞下颌关节（TMJ）炎症性疾病的多种促炎介质之一，由诱导型一氧化氮合酶（iNOS）合成。在炎症、增殖和凋亡情况下，iNOS受核因子-κB（NF-κB）转录调控。据报道，在膝关节中，一氧化氮受角叉菜胶正向调节，受透明质酸负向调节。本研究的目的是通过研究iNOS、NF-κB和抗增殖细胞核抗原（PCNA）的免疫反应性，从组织学角度评估在大鼠TMJ中外源性给予角叉菜胶和透明质酸如何影响滑膜的炎症和细胞增殖。结果显示，iNOS、NF-κB和PCNA的免疫反应性细胞通常仅正常定位于野生型TMJ的滑膜中。在表现出过度折叠的角叉菜胶注射滑膜中，免疫反应性细胞数量大量增加，而在显示少量褶皱的透明质酸注射滑膜中，免疫反应性细胞数量较少。这些结果表明，角叉菜胶注射尤其在滑膜中诱导了炎症和细胞增殖，而透明质酸通过减少滑膜中的炎症分子缓解了炎症。
