Institute of Food Biotechnology and Food Chemistry, Berlin University of Technology, Germany.
Appl Biochem Biotechnol. 2011 Jun;164(4):443-53. doi: 10.1007/s12010-010-9146-2. Epub 2010 Dec 29.
Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growth and secondary metabolism, particularly anthocyanins and phenolic acids synthesis, were investigated by using suspension culture of Vitis vinifera L. cv. Gamay Fréaux as a model system. Anthocyanins were measured by spectrophotometer and extracellular phenolic acids were determined by high-performance liquid chromatography. The compounds were identified by liquid chromatography-mass spectrometry and nuclear magnetic resonance. After the treatments with PEF and ethephon, the concentrations of anthocyanins and phenolic acids in cell culture were higher than in the control, without loss of biomass. The combination of PEF treatment and ethephon improved secondary metabolites formation. Production levels of extracellular phenolic acids, 3-O-glucosyl-resveratrol were increased by PEF and ethephon treatments. The results show that PEF induced a defense response of plant cells and may have altered the cell/membrane's dielectric properties. PEF, an external stimulus or stress, is proposed as a promising new abiotic elicitor for stimulating secondary metabolites biosynthesis in plant cell cultures.
植物细胞培养为次生代谢产物的生产提供了巨大的潜力。通过应用不同的物理和化学细胞胁迫因素，我们研究了植物细胞培养中次生代谢产物的生产。使用葡萄悬浮培养物作为模型系统，研究了脉冲电场 (PEF) 和乙烯利对生长和次生代谢物（特别是花青素和酚酸合成）的影响。花青素用分光光度计测量，细胞外酚酸用高效液相色谱法测定。通过液相色谱-质谱和核磁共振鉴定了化合物。PEF 和乙烯利处理后，细胞培养物中花青素和酚酸的浓度高于对照，而生物量没有损失。PEF 处理与乙烯利的结合改善了次生代谢产物的形成。PEF 和乙烯利处理增加了细胞外酚酸、3-O-葡萄糖基白藜芦醇的产量。结果表明，PEF 诱导了植物细胞的防御反应，可能改变了细胞膜的介电特性。PEF 作为一种外部刺激或胁迫，被提议作为一种有前途的新非生物诱导子，用于刺激植物细胞培养物中次生代谢产物的生物合成。
