Institute of Food Biotechnology and Food Chemistry, Berlin University of Technology, Königin-Luise Str. 22, 14195 Berlin, Germany.
Plant Cell Rep. 2012 Mar;31(3):461-77. doi: 10.1007/s00299-011-1165-0. Epub 2011 Oct 11.
This review addresses methods of obtaining secondary metabolites from plant cell suspension and hairy root cultures and their exudates, particularly the physiological mechanisms of secondary metabolites release and trafficking. The efficiency for product recovery of metabolites can be increased by various methods, based on the principle of continuous product release into the cultivation medium. The most common methods for metabolite recovery are elicitation, influencing membrane permeability, and in situ product removal. The biosynthetic pathways can be influenced by cultivation conditions, transformation, or application of elicitors. The membrane permeability can be altered through the application of chemical or physical treatments. Product removal can be greatly increased through a two-phase system and the introduction of absorbents into the cultivation medium. In this review, we describe some improved approaches that have proven useful in these efforts.
本综述探讨了从植物细胞悬浮培养物和发根培养物及其渗出液中获得次生代谢产物的方法，特别是次生代谢产物释放和运输的生理机制。基于产物连续释放到培养介质中的原理，可以通过各种方法提高代谢产物的回收效率。最常见的代谢产物回收方法是诱导、影响膜通透性和原位产物去除。生物合成途径可以通过培养条件、转化或诱导剂的应用来影响。可以通过化学或物理处理来改变膜通透性。通过两相系统和将吸收剂引入培养介质，可以大大增加产物的去除率。在本综述中，我们描述了一些经过验证的有用的改进方法。
