镍（II）对膨胀活性污泥性能的影响及利用 16S rDNA 基因对污泥微生物分析。
The effect of Ni(II) on properties of bulking activated sludge and microbial analysis of sludge using 16S rDNA gene.
机构信息
Department of Environmental Science and Engineering, Fudan University, Shanghai 200433, China.
出版信息
Bioresour Technol. 2011 Feb;102(4):3783-9. doi: 10.1016/j.biortech.2010.12.022. Epub 2010 Dec 9.
This study investigated the effect of nickel on properties and microbial community of bulking activated sludge when 60-240 mg/L Ni(II) was dosed continuously in a sequencing batch reactor (SBR) over 350 days. Results showed that 120 mg/L nickel did not significantly inhibited removal of organic pollutant by activated sludge. However, the system was completely upset when 240 mg/L Ni(II) was dosed. Improvement of settling and dewatering ability was also observed with the addition of Ni(II). In addition, investigations by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA of bacteria strain demonstrated that Ni(II) significantly affected microbial community of bulking activated sludge, judging from the elimination of original species and emergence of possible new nickel-resistant bacteria. The effect of nickel on shift of microbial community was an important cause resulted in the improvement of sludge properties in this bulking activated sludge system.
本研究考察了镍对膨胀活性污泥性能和微生物群落的影响，在序批式反应器（SBR）中连续投加 60-240mg/L 的 Ni（II），持续 350 天。结果表明，120mg/L 的镍对活性污泥去除有机污染物没有明显抑制作用。然而，当投加 240mg/L Ni（II）时，系统完全崩溃。添加 Ni（II）也改善了沉降和脱水能力。此外，通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）对细菌 16S rDNA 的研究表明，Ni（II）显著影响了膨胀活性污泥的微生物群落，从原始物种的消除和可能的新耐镍细菌的出现可以判断。镍对微生物群落的转移的影响是导致该膨胀活性污泥系统中污泥性能改善的重要原因。
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