利用 16S rDNA 和镍抗性基因对活性污泥中的耐镍微生物群落进行特征描述和定量分析。
Characterization and quantification of the nickel resistant microbial community in activated sludge using 16S rDNA and nickel resistance genes.
机构信息
Environmental Biotechnology Laboratory, Department of Civil Engineering, University of Hong Kong, Hong Kong, China.
出版信息
Environ Technol. 2011 Apr;32(5-6):533-42. doi: 10.1080/09593330.2010.504749.
The effect of nickel on the microbial community in the activated sludge of a sequencing batch reactor (SBR) reactor was investigated by continuously dosing nickel from 60 to 240 mg Ni(II) L(-1). The diversity of the microbial community was investigated by polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis of the variable V3 region of the bacterial 16S rDNA. The experimental results showed that the community structure changed significantly after dosing with nickel with a shift in dominant species, the disappearance of some original species and the emergence of some new species. The existence of a nickel resistant gene was also investigated using PCR. The obtained nickel resistance gene had a maximum homology with the plasmid pMOL30 of Ralstonia metallidurans CH34. The quantitative real-time PCR results indicated that the quantity of the nickel resistance gene was related to the nickel concentration loaded to the reactor.
采用连续投加镍的方式，将序批式活性污泥法（SBR）中的镍浓度从 60mg/L 提升至 240mg/L，研究镍对活性污泥微生物群落的影响。通过聚合酶链式反应（PCR）和细菌 16S rDNA 的可变 V3 区变性梯度凝胶电泳，研究微生物群落的多样性。实验结果表明，投加镍后，群落结构发生了明显变化，优势种群发生了转变，一些原有种群消失，出现了一些新种群。还利用 PCR 研究了镍抗性基因的存在。获得的镍抗性基因与 Ralstonia metallidurans CH34 的质粒 pMOL30 具有最大同源性。实时定量 PCR 结果表明，镍抗性基因的数量与反应器中加载的镍浓度有关。
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