Govoreanu R, Seghers D, Nopens I, De Clercq B, Saveyn H, Capalozza C, Van der Meeren P, Verstraete W, Top E, Vanrolleghem P A
BIOMATH, Ghent University, Coupure Links 653, Ghent, Belgium.
Water Sci Technol. 2003;47(12):9-18.
Over a period of 227 days properties of activated sludge grown in an sequencing batch reactor (SBR) operated under stable conditions were analyzed. Settling properties (sludge volume index (SVI)) of the activated sludge were compared with on-line measurements of floc size and size distribution obtained by using a laser light scattering technique (Malvern Mastersizer/S, Malvern, UK), and with measurements of microbial community dynamics analyzed by denaturing gradient gel electrophoresis (DGGE) patterns of 16S rRNA genes. In addition, microscopical observations were used to confirm the results. Three distinct stages in the SBR evolution were observed. In the first stage the structural floc properties showed predominant presence of floc-forming bacteria in the activated sludge. A good correlation between floc size, properties and microbial community evolution was observed. The second stage showed a good balance between floc-forming and filamentous bacteria, with good settling properties and a highly dynamic community in the SBR. In the third stage, an increase in the filamentous bacteria, which became predominant in the system was observed. Again, a good correlation between settling properties and floc size distribution was obtained and a new dominant species was observed in the DGGE patterns, which can be assumed to be a filamentous organism.
在227天的时间里，对在稳定条件下运行的序批式反应器（SBR）中生长的活性污泥的特性进行了分析。将活性污泥的沉降特性（污泥体积指数（SVI））与使用激光散射技术（英国马尔文的Malvern Mastersizer/S）获得的絮体大小和大小分布的在线测量结果进行比较，并与通过16S rRNA基因的变性梯度凝胶电泳（DGGE）图谱分析的微生物群落动态测量结果进行比较。此外，还通过显微镜观察来证实结果。观察到SBR演变过程中有三个不同阶段。在第一阶段，结构絮体特性表明活性污泥中主要存在絮凝形成菌。观察到絮体大小、特性与微生物群落演变之间存在良好的相关性。第二阶段显示絮凝形成菌和丝状菌之间达到良好平衡，SBR具有良好的沉降特性和高度动态的群落。在第三阶段，观察到丝状菌增加并在系统中占主导地位。同样，沉降特性与絮体大小分布之间获得了良好的相关性，并且在DGGE图谱中观察到一个新的优势物种，可以假定为丝状生物。
