Austen B M, Haberland M E, Nyc J F, Smith E L
J Biol Chem. 1977 Nov 25;252(22):8142-9.
A sequence is presented for the COOH-terminal 669 residues of the NAD-specific glutamate dehydrogenase of Neurospora crassa. Comparison of this sequence with those of the vertebrate glutamate dehydrogenases of chicken and bovine liver and with the NADP-specific enzyme of Neurospora shows some similarities in sequences around residues previously identified as important for the function of these enzymes. These are: (a) the reactive lysine residue of low pK in the NADP and the vertebrate enzymes; (b) the tyrosine residue of the NADP enzyme that is readily nitrated by tetranitromethane with inactivation, a residue protected by NADP or by NMN; and (c) the arginine residue of the NADP-enzyme that is reactive with 1,2-cyclohexanedione with inactivation. Despite these similarities, comparison of the sequence of the NAD-enzyme with those of the other glutamate dehydrogenases of known sequences revealed relatively little overall homology as determined by computer analysis.
本文给出了粗糙脉孢菌NAD特异性谷氨酸脱氢酶COOH末端669个残基的序列。将该序列与鸡和牛肝的脊椎动物谷氨酸脱氢酶以及脉孢菌的NADP特异性酶的序列进行比较，发现在先前确定对这些酶的功能很重要的残基周围的序列存在一些相似性。这些相似性包括：（a）NADP和脊椎动物酶中低pK的反应性赖氨酸残基；（b）NADP酶中易被四硝基甲烷硝化并导致失活的酪氨酸残基，该残基受NADP或NMN保护；（c）NADP酶中与1,2 - 环己二酮反应并导致失活的精氨酸残基。尽管存在这些相似性，但通过计算机分析确定，NAD酶的序列与其他已知序列的谷氨酸脱氢酶的序列相比，总体同源性相对较低。
