Haberland M E, Smith E L
J Biol Chem. 1977 Nov 25;252(22):8196-205.
The isolation and sequences of several large peptides from cyanogen bromide cleavage of the 1030-residue polypeptide chain of the NAD-specific glutamate dehydrogenase of Neurospora crassa are described. One of these is in the 669-residue sequence of the COOH-terminal end of the protein. The remaining peptides have been aligned in two partial sequences in the NH2-terminal portion of the polypeptide chain.
本文描述了从粗糙脉孢菌NAD特异性谷氨酸脱氢酶1030个残基的多肽链经溴化氰裂解后分离得到的几种大肽段及其序列。其中一个位于该蛋白质COOH末端的669个残基序列中。其余肽段已在多肽链的NH2末端部分排列成两个部分序列。