Austen B M, Nyc J F, Degani Y, Smith E L
Proc Natl Acad Sci U S A. 1975 Dec;72(12):4891-4. doi: 10.1073/pnas.72.12.4891.
Parts of the primary structure of the NAD-specific glutamate dehydrogenase [L-glutamate:NAD oxidoreductase (deaminating), EC 1.4.1.2] from Neurospora crassa are presented. Segments of the sequence representing 886 unique amino-acid residues have been determined; the largest contains 267 residues. There are only short regions of possible homology between this enzyme and the glutamate dehydrogenases of bovine liver or the NADP-specific enzyme of Neurospora. The large size of the subunit (116,000 molecular weight) of the NAD-specific glutamate dehydrogenase is unusual when compared to other known dehydrogenases.
本文展示了粗糙脉孢菌中NAD特异性谷氨酸脱氢酶[L-谷氨酸:NAD氧化还原酶(脱氨基),EC 1.4.1.2]一级结构的部分内容。已确定代表886个独特氨基酸残基的序列片段;最大的片段包含267个残基。该酶与牛肝谷氨酸脱氢酶或粗糙脉孢菌的NADP特异性酶之间仅存在可能的短同源区域。与其他已知脱氢酶相比,NAD特异性谷氨酸脱氢酶的亚基(分子量116,000)尺寸较大,这很不寻常。