Schmidt A, Sandmann G
Lehrstuhl für Biochemie und Physiologie der Pflanzen, Universität Konstanz, F.R.G.
Gene. 1990 Jul 2;91(1):113-7. doi: 10.1016/0378-1119(90)90170-v.
The membrane-bound phytoene dehydrogenase (PD) is an enzyme in carotenoid biosynthesis which is essential in all microorganisms and plants containing these colored pigments. Despite its key role in the regulation of carotenogenesis, the biochemistry and molecular biology of PD are poorly understood. We have cloned, sequenced and expressed a portion of the PD-encoding gene, crtI, from the blue-green algae Aphanocapsa PCC6714. The gene codes for a 532-amino acids (aa) protein, with a calculated Mr of 52,598 Da. Two regions of the aa sequence share significant homology with PD from the purple bacterium Rhodobacter capsulatus, including a 30-aa region which has been proposed to be specific for dehydrogenases in carotenoid biosynthesis.
膜结合的八氢番茄红素脱氢酶（PD）是类胡萝卜素生物合成中的一种酶，在所有含有这些有色色素的微生物和植物中都至关重要。尽管其在类胡萝卜素生成调控中起关键作用，但对PD的生物化学和分子生物学了解甚少。我们已从蓝藻集胞藻PCC6714中克隆、测序并表达了部分编码PD的基因crtI。该基因编码一个532个氨基酸（aa）的蛋白质，计算分子量为52,598 Da。氨基酸序列的两个区域与紫色细菌荚膜红细菌的PD具有显著同源性，包括一个30个氨基酸的区域，该区域被认为是类胡萝卜素生物合成中脱氢酶所特有的。
