MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 0XZ, UK.
J Mol Biol. 2011 Feb 25;406(3):355-61. doi: 10.1016/j.jmb.2010.12.035. Epub 2010 Dec 30.
MCM3 acetylase (MCM3AP) and germinal-centre associated nuclear protein (GANP) are transcribed from the same locus and are therefore confused in databases because the MCM3 acetylase DNA sequence is contained entirely within the much larger GANP sequence and the entire MCM3AP sequence is identical to the carboxy terminus of GANP. Thus, the MCM3AP and GANP genes are read in the same reading frame and MCM3AP is an N-terminally truncated region of GANP. However, we show here that MCM3AP and GANP are different proteins, occupying different locations in the cell and transcribed from different promoters. Intriguingly, a promoter for MCM3AP lies within an intron of GANP. This report is an interesting example in nature of two separate gene products from the same locus that perform two entirely different functions in the cell. Therefore, to avoid further confusion, they should now be referred to as separate but overlapping genes.
MCM3 乙酰基转移酶（MCM3AP）和生发中心相关核蛋白（GANP）由同一个基因座转录而来，因此在数据库中容易混淆，因为 MCM3 乙酰基转移酶的 DNA 序列完全包含在更大的 GANP 序列中，而整个 MCM3AP 序列与 GANP 的羧基末端完全相同。因此，MCM3AP 和 GANP 基因以相同的阅读框读取，MCM3AP 是 GANP 的 N 端截断区域。然而，我们在这里表明，MCM3AP 和 GANP 是不同的蛋白质，它们在细胞中占据不同的位置，并且由不同的启动子转录。有趣的是，MCM3AP 的启动子位于 GANP 的内含子中。本报告是自然界中一个有趣的例子，即同一个基因座的两个不同的基因产物在细胞中执行两种完全不同的功能。因此，为了避免进一步混淆，它们现在应该被称为独立但重叠的基因。
