组织特异性转录调控在人类和小鼠之间已经有了显著的分化。
Tissue-specific transcriptional regulation has diverged significantly between human and mouse.
作者信息
Odom Duncan T, Dowell Robin D, Jacobsen Elizabeth S, Gordon William, Danford Timothy W, MacIsaac Kenzie D, Rolfe P Alexander, Conboy Caitlin M, Gifford David K, Fraenkel Ernest
机构信息
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA.
出版信息
Nat Genet. 2007 Jun;39(6):730-2. doi: 10.1038/ng2047. Epub 2007 May 21.
Abstract
We demonstrate that the binding sites for highly conserved transcription factors vary extensively between human and mouse. We mapped the binding of four tissue-specific transcription factors (FOXA2, HNF1A, HNF4A and HNF6) to 4,000 orthologous gene pairs in hepatocytes purified from human and mouse livers. Despite the conserved function of these factors, from 41% to 89% of their binding events seem to be species specific. When the same protein binds the promoters of orthologous genes, approximately two-thirds of the binding sites do not align.
摘要
我们证明，高度保守的转录因子的结合位点在人类和小鼠之间存在广泛差异。我们将四种组织特异性转录因子（FOXA2、HNF1A、HNF4A和HNF6）与从人类和小鼠肝脏中纯化的肝细胞中的4000对直系同源基因对的结合进行了定位。尽管这些因子具有保守功能，但其41%至89%的结合事件似乎具有物种特异性。当同一蛋白质与直系同源基因的启动子结合时，大约三分之二的结合位点并不对齐。
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