Oral & Maxillofacial Pathology Department, Dental Research Center, Faculty of Dentistry, Mashhad University of Medical Sciences, Iran.
Med Oral Patol Oral Cir Bucal. 2011 Sep 1;16(6):e730-5. doi: 10.4317/medoral.17007.
In oral cavity, the tongue is the most common site prone to development of squamous cell carcinoma (SCC). Considering malignant transformation as a cellular stress, the expression of heat shock proteins (HSPs) may be affected in this process. In this study we assessed the expression of HSP105 and HSP27 as two of the most interested stress proteins and investigated their relationship with grade and stage of the tongue SCC.
Fifty-six specimens including 31 early and 25 advanced tongue SCC were gathered. All specimens were graded histologically from I to III. Sixteen sections of normal oral mucosa were used as control group. The cellularity and intensity of HSP105 and HSP27 expression were studied immunohistochemically in both case and control groups. Results were expressed by histochemical score (HSCORE).
Significant differences were observed between expression of HSPs and stage of the disease. From early to advanced stage, the expression of HSP105 and HSP27 increased and decreased, respectively. There was no relationship between histological grade of lesion and HSCORE of HSP105 expression (P=0.5), although, HSP27 expression had reverse relationship with the SCC histological grade.
HSP27 and HSP105 may be indicated for prognostic purposes in evaluation of tongue SCC. HSP 27 may be used for more accurate microscopic grading of tongue SCC. Increased expression of HSP105 in advanced stage may lead to using this protein for immunotherapy of tongue SCC.
口腔中，舌是最常见的易于发生鳞状细胞癌（SCC）的部位。考虑到恶性转化是一种细胞应激，热休克蛋白（HSPs）的表达可能在此过程中受到影响。在这项研究中，我们评估了 HSP105 和 HSP27 的表达，作为最感兴趣的两种应激蛋白之一，并研究了它们与舌 SCC 的分级和分期的关系。
收集了包括 31 例早期和 25 例晚期舌 SCC 在内的 56 个标本。所有标本均按组织学分级为 I 至 III 级。将 16 个正常口腔黏膜切片作为对照组。在病例和对照组中均通过免疫组织化学研究 HSP105 和 HSP27 的细胞表达强度。结果用组织化学评分（HSCORE）表示。
HSPs 的表达与疾病的分期之间存在显著差异。从早期到晚期，HSP105 和 HSP27 的表达分别增加和减少。HSP105 表达的 HSCORE 与病变的组织学分级之间无相关性（P=0.5），尽管 HSP27 的表达与 SCC 的组织学分级呈负相关。
HSP27 和 HSP105 可用于评估舌 SCC 的预后。HSP27 可用于更准确地进行舌 SCC 的显微镜分级。晚期 HSP105 表达增加可能导致将该蛋白用于舌 SCC 的免疫治疗。
