Virology Unit, Department of Public Health and Microbiology, Turin, Italy.
Minerva Med. 2010 Dec;101(6):385-9.
The recently described polyomaviruses KI and WU have been detected in respiratory samples, stools, tonsils, and blood, particularly in immunocompromised conditions, although little is known about tissue tropism. Herein we investigated the occurrence of KIV and WUV in non-malignant tonsillar specimens by Real-time quantitative PCR; the presence of polyomaviruses BK, JC and SV40-DNA was also evaluated.
Twenty-nine non-malignant tonsil specimens obtained from children and adults admitted for tonsillectomy were prospectively studied. Real-time quantitative TaqMan PCR for polyomaviruses KI, WU, BK, JC, and SV40 were performed.
KI-DNA was positive in 2/29 tonsillar specimens (6.9%), while BK- DNA, JC-DNA, SV-40 DNA, and WU-DNA sequences were not identified.
Few studies have investigated the prevalence of polyomaviruses in tonsil specimens, with varying results, and data are particularly scant as regards the newly discovered KIV and WUV. Two major questions remain to be definitely answered at this regard: the possibility that human tonsils represent the initial site of infection and/or a latency site and the biological and clinical meaning of KIV and WUV in different contexts and groups of patients, in that it is not clear whether they are simple bystanders or play a role in tonsil disease.
最近描述的多瘤病毒 KI 和 WU 已在呼吸道样本、粪便、扁桃体和血液中被检测到，尤其是在免疫功能低下的情况下，尽管对组织嗜性知之甚少。在此，我们通过实时定量 PCR 研究了非恶性扁桃体标本中 KIV 和 WUV 的发生情况；还评估了多瘤病毒 BK、JC 和 SV40-DNA 的存在情况。
前瞻性研究了 29 例因扁桃体切除术而入院的儿童和成人的非恶性扁桃体标本。对多瘤病毒 KI、WU、BK、JC 和 SV40 进行了实时定量 TaqMan PCR。
29 份扁桃体标本中有 2 份（6.9%）KI-DNA 阳性，而 BK-DNA、JC-DNA、SV-40 DNA 和 WU-DNA 序列未被识别。
少数研究调查了多瘤病毒在扁桃体标本中的流行情况，结果各不相同，而关于新发现的 KIV 和 WUV 的数据则特别稀少。在这方面，有两个主要问题需要明确回答：人类扁桃体是否可能是感染的初始部位和/或潜伏部位，以及 KIV 和 WUV 在不同情况下和不同患者群体中的生物学和临床意义，因为尚不清楚它们是单纯的旁观者还是在扁桃体疾病中发挥作用。
