Saito K
Department of Bioscience and Technology, School of Engineering, Hokkaido Tokai University, Sapporo, Japan.
Biochim Biophys Acta. 1990 Sep 14;1035(3):340-7. doi: 10.1016/0304-4165(90)90098-h.
It was demonstrated that 8-C-beta-D-glucopyranosyl-3',4',5,7-tetrahydroxy- flav-2-en-3-one (orientin) undergoes enzyme-catalyzed deglucosylation to form the sugar-free aglycone, 3',4',5,7-tetrahydroxyflav- 2-en-3-one (luteolin). The enzyme was partially purified by ammonium sulfate precipitation, followed by fractionation through Sephadex G-100 gel. The enzymic deglycosylation proceeded at pH 5.4 in an incubation system containing enzyme preparation isolated from Carthamus tinctorius and orientin, with no distinct requirement for atmospheric oxygen. It was activated markedly by the addition of H2O2 and inhibited prominently in the presence of iron ions, KCN or o-phenanthroline.
已证明8 - C - β - D - 吡喃葡萄糖基 - 3',4',5,7 - 四羟基黄酮 - 2 - 烯 - 3 - 酮（荭草素）经酶催化去糖基化反应形成无糖苷元，即3',4',5,7 - 四羟基黄酮 - 2 - 烯 - 3 - 酮（木犀草素）。该酶通过硫酸铵沉淀进行部分纯化，随后通过Sephadex G - 100凝胶进行分级分离。酶促去糖基化反应在pH 5.4的孵育体系中进行，该体系含有从红花中分离得到的酶制剂和荭草素，对大气中的氧气无明显需求。添加H2O2可显著激活该酶，而在铁离子、KCN或邻菲罗啉存在的情况下则受到显著抑制。
