Jiang J W, Zhong C S, Yu Y F, Wei C C, Xu Z Y
Department of Biophysics, Faculty of Basic Medical Science, Hua Shan Hospital, Shanghai Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1990;8(2):84-7.
8 rabbits were infected percutaneously with the cercariae of Schistosoma japonicum. After hepatic fibrosis had developed in these rabbits 4 months after infection, 4 out of the 8 infected rabbits were given colchicine orally at a dosage of 40 micrograms/kg per day for 7 weeks. Another uninfected rabbits were used as controls. The therapeutic effects of colchicine on hepatic fibrosis were studied by transmission electron microscopy and morphometry, in which the area of collagen microfibrils in the space of disse and liver cells was measured. The results showed that colchicine relieved ultrastructural injury of liver cells and reduced the number of active fibroblasts and collagen microfibrils. The area of collagen microfibrils measured in the liver of the infected rabbits and colchicine-treated rabbits accounted for 42.5% and 0.2%, respectively (P less than 0.01), suggesting that colchicine has therapeutic effect against schistosomal liver fibrosis.
8只兔子经皮感染日本血吸虫尾蚴。感染4个月后这些兔子出现肝纤维化，8只感染兔子中有4只每天按40微克/千克的剂量口服秋水仙碱，持续7周。另取未感染的兔子作为对照。通过透射电子显微镜和形态测量学研究秋水仙碱对肝纤维化的治疗效果，测量狄氏间隙和肝细胞中胶原微纤维的面积。结果显示，秋水仙碱减轻了肝细胞的超微结构损伤，减少了活跃成纤维细胞和胶原微纤维的数量。感染兔子肝脏和经秋水仙碱治疗兔子肝脏中测得的胶原微纤维面积分别占42.5%和0.2%（P小于0.01），表明秋水仙碱对血吸虫性肝纤维化有治疗作用。
