用单克隆抗体通过亲和层析法分离克氏锥虫抗原。对其在血清学检测中可能应用的初步评估。

Carbonetto C H, Malchiodi E L, Chiaramonte M, Durante de Isola E, Fossati C A, Margni R A

Institute of Humoral Immunity Studies (IDEHU) CONICET/UBA, Department of Immunology, Faculty of Pharmacology and Biochemistry, Argentina.

Clin Exp Immunol. 1990 Oct;82(1):93-6. doi: 10.1111/j.1365-2249.1990.tb05409.x.

By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas' disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.

通过使用我们实验室获得的单克隆抗体（163B6）进行亲和层析，我们分离出了一种克氏锥虫抗原，该抗原可能有助于从利什曼病中鉴别诊断恰加斯病。在酶联免疫吸附测定（ELISA）中，这种52-kD的抗原与所有检测的恰加斯病患者血清发生反应，但不与利什曼病患者血清发生反应。由于163B6单克隆抗体与兰氏锥虫以及克氏锥虫前鞭毛体的8个菌株和1个克隆发生反应，所以52-kD抗原在锥虫属中广泛分布。