• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

聚丙烯酰胺凝胶中蛋白质的超敏染色显示脑脊液蛋白质存在区域差异。

Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.

作者信息

Merril C R, Goldman D, Sedman S A, Ebert M H

出版信息

Science. 1981 Mar 27;211(4489):1437-8. doi: 10.1126/science.6162199.

DOI:10.1126/science.6162199
PMID:6162199
Abstract

A new silver stain for electrophoretically separated polypeptides can be rapidly and easily used and can detect as little as 0.01 nanogram of protein per square millimeter. When employed with two-dimensional electrophoresis, it should permit qualitative and quantitative characterization of protein distributions in body fluids and tissues. It has been used to demonstrate regional variations in cerebrospinal fluid proteins.

摘要

一种用于电泳分离多肽的新型银染法操作迅速简便,每平方毫米能检测低至0.01纳克的蛋白质。与二维电泳结合使用时,它应能对体液和组织中的蛋白质分布进行定性和定量表征。它已被用于证明脑脊液蛋白质的区域差异。

相似文献

1
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.聚丙烯酰胺凝胶中蛋白质的超敏染色显示脑脊液蛋白质存在区域差异。
Science. 1981 Mar 27;211(4489):1437-8. doi: 10.1126/science.6162199.
2
"Ultrasensitive" silver stains: their use exemplified in the study of normal human cerebrospinal fluid proteins separated by two-dimensional electrophoresis.“超敏”银染法:二维电泳分离正常人脑脊液蛋白质研究中的应用实例
Clin Chem. 1984 Dec;30(12 Pt 1):1938-42.
3
Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4335-9. doi: 10.1073/pnas.76.9.4335.
4
Optimal detection of oligoclonal bands in CSF by silver stain.通过银染法对脑脊液中寡克隆带进行最佳检测。
Neurology. 1983 Nov;33(11):1510-2. doi: 10.1212/wnl.33.11.1510.
5
A sensitive urea-silver stain method for detecting trace quantities of separated proteins in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中分离蛋白质痕量的灵敏尿素银染法。
Prep Biochem. 1987;17(1):93-9. doi: 10.1080/00327488708062478.
6
A silver staining method for protein bands or immunoprecipitates in electrophoresis or immunoelectrophoretic treatment of unconcentrated cerebrospinal fluid proteins using the commercial agarose plates (Corning Universal System).一种使用商用琼脂糖平板(康宁通用系统)对未浓缩脑脊液蛋白质进行电泳或免疫电泳处理时,用于蛋白质条带或免疫沉淀物的银染方法。
J Neurosci Methods. 1982 Mar;5(3):221-6. doi: 10.1016/0165-0270(82)90072-3.
7
A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中蛋白质的简化超灵敏银染法。
Anal Biochem. 1980 Jul 1;105(2):361-3. doi: 10.1016/0003-2697(80)90470-4.
8
Two-dimensional electrophoresis and "ultrasensitive" silver staining of cerebrospinal fluid proteins in neurological diseases.神经疾病中脑脊液蛋白质的二维电泳及“超敏”银染色
Clin Chem. 1984 Dec;30(12 Pt 1):1933-7.
9
Horizontal two-dimensional electrophoresis of cerebrospinal fluid proteins with immobilized pH gradients in the first dimension.脑脊液蛋白质的水平二维电泳,第一维采用固定化pH梯度。
Electrophoresis. 1990 Jan;11(1):91-2. doi: 10.1002/elps.1150110118.
10
Silver stain for proteins in ultrathin polyacrylamide gels: a sensitive precipitation technique.超薄聚丙烯酰胺凝胶中蛋白质的银染法:一种灵敏的沉淀技术。
Anal Biochem. 1988 Nov 1;174(2):589-92. doi: 10.1016/0003-2697(88)90060-7.

引用本文的文献

1
"Proteinjury": a universal pathological mechanism mediated by cerebrospinal fluid in neurodegeneration and trauma.“蛋白质损伤”:一种由脑脊液介导的、在神经退行性变和创伤中普遍存在的病理机制。
Front Cell Dev Biol. 2025 May 20;13:1593122. doi: 10.3389/fcell.2025.1593122. eCollection 2025.
2
Evaluation of different glycerol fed-batch strategies in a lab-scale bioreactor for the improved production of a novel engineered β-fructofuranosidase enzyme in Pichia pastoris.评价在实验室规模生物反应器中不同甘油流加策略对新型毕赤酵母工程 β-呋喃果糖苷酶生产的影响。
World J Microbiol Biotechnol. 2024 May 31;40(7):223. doi: 10.1007/s11274-024-04027-6.
3
Enzyme variants in biosynthesis and biological assessment of different molecular weight hyaluronan.
不同分子量透明质酸生物合成及生物学评估中的酶变体
AMB Express. 2024 May 10;14(1):56. doi: 10.1186/s13568-024-01713-4.
4
Sperm motility initiation factor is a minor component of the Pacific herring egg chorion.精子活力起始因子是太平洋鲱鱼卵绒毛膜的一种次要成分。
Dev Growth Differ. 1996 Apr;38(2):193-202. doi: 10.1046/j.1440-169X.1996.t01-1-00009.x.
5
Analysis of the enzymatic degradation of lysozyme fibrils using a combination method of non-denaturing gel electrophoresis and double staining with Coomassie Brilliant Blue G-250 and R-250 dyes.采用非变性凝胶电泳以及考马斯亮蓝G-250和R-250染料双重染色的组合方法对溶菌酶原纤维的酶促降解进行分析。
Anal Sci. 2023 Mar;39(3):267-274. doi: 10.1007/s44211-022-00229-w. Epub 2022 Nov 30.
6
Human cytomegalovirus UL24 and UL43 products participate in SAMHD1 subcellular localization.人巨细胞病毒UL24和UL43产物参与SAMHD1的亚细胞定位。
Virusdisease. 2022 Dec;33(4):383-396. doi: 10.1007/s13337-022-00799-3. Epub 2022 Nov 11.
7
Rat Group IIA Secreted Phospholipase A Binds to Cytochrome c Oxidase and Inhibits Its Activity: A Possible Episode in the Development of Alzheimer's Disease.大鼠IIA组分泌型磷脂酶A与细胞色素c氧化酶结合并抑制其活性:阿尔茨海默病发展过程中的一个可能事件。
Int J Mol Sci. 2022 Oct 15;23(20):12368. doi: 10.3390/ijms232012368.
8
Thurincin H Is a Nonhemolytic Bacteriocin of Bacillus thuringiensis with Potential for Applied Use.苏云金菌素 H 是一种具有应用潜力的非溶血苏云金芽孢杆菌细菌素。
Probiotics Antimicrob Proteins. 2023 Aug;15(4):955-966. doi: 10.1007/s12602-022-09952-2. Epub 2022 May 24.
9
Modification in Silver Staining Procedure for Enhanced Protein Staining.改良银染法增强蛋白质染色效果
Biomed Res Int. 2022 May 13;2022:6243971. doi: 10.1155/2022/6243971. eCollection 2022.
10
Extraction and Electrophoretic Analysis of Bacterial Lipopolysaccharides and Outer Membrane Proteins.细菌脂多糖和外膜蛋白的提取及电泳分析
Bio Protoc. 2021 Dec 20;11(24):e4263. doi: 10.21769/BioProtoc.4263.