采用多重实时 PCR 检测肠道病原体的方法评价一种简化的 QIAsymphony DNA 粪便提取方案。
Evaluation of a shortened QIAsymphony DNA extraction protocol for stool samples using a multiplex real-time PCR for the detection of enteric pathogens.
机构信息
Laboratory for Infectious Diseases, Department of Research and Development, Groningen, The Netherlands.
出版信息
J Microbiol Methods. 2011 Feb;84(2):365-7. doi: 10.1016/j.mimet.2010.12.023. Epub 2010 Dec 31.
PMID:21199665
Abstract
A shortened DNA extraction protocol for the QIAsymphony SP was evaluated by quantitative and qualitative comparison of real-time PCR results of 150 co-extracted stool samples. The average ∆Cycle threshold value for positive pathogenic targets was 0.28 Ct. A consensus of 96.91%, with a correlation coefficient of 0.9880 was recorded.
摘要
对 QIAsymphony SP 的 DNA 提取方案进行了简化，通过对 150 个共提取粪便样本的实时 PCR 结果的定量和定性比较进行了评估。阳性致病目标的平均 ∆Cycle threshold 值为 0.28 Ct。记录了 96.91%的一致性，相关系数为 0.9880。
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