比较 QIAsymphony 自动化核酸提取和 PCR 设定平台与 NucliSens easyMAG 和 Corbett CAS-1200 液体处理工作站，用于检测粪便样本中的肠道病原体。
Comparison of the QIAsymphony automated nucleic acid extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples.
机构信息
Laboratory for Infectious Diseases, Department of Research and Development, Groningen, The Netherlands.
出版信息
J Microbiol Methods. 2011 Feb;84(2):335-40. doi: 10.1016/j.mimet.2010.12.024. Epub 2010 Dec 31.
In this study we compared the QIAsymphony Sample Preparation and Assay Setup modules (Qiagen), which provide automated nucleic acid extraction and PCR setup, to the NucliSens easyMAG (bioMérieux) and CAS-1200 liquid handling station (Corbett) for a molecular screening approach of enteric pathogens in fecal samples using multiplex real-time PCR. The relative DNA recovery of both platforms, within- and between-run reproducibility and a prospective study, including 510 clinical fecal samples, were performed. The results demonstrated that the QIAsymphony Sample Preparation and Assay Setup modules were highly reproducible and achieve equal performance, quantitative and qualitative, when compared with the NucliSens easyMAG and CAS-1200 systems for the molecular screening analysis of enteric pathogens in fecal samples.
在这项研究中，我们比较了 QIAsymphony Sample Preparation 和 Assay Setup 模块（Qiagen），该模块提供自动化核酸提取和 PCR 设定，以及 NucliSens easyMAG（bioMérieux）和 CAS-1200 液体处理站（Corbett），用于使用多重实时 PCR 对粪便样本中的肠道病原体进行分子筛选。我们进行了两个平台的相对 DNA 回收率、批内和批间重现性以及包括 510 份临床粪便样本的前瞻性研究。结果表明，QIAsymphony Sample Preparation 和 Assay Setup 模块具有高度重现性，并且在对粪便样本中的肠道病原体进行分子筛选分析时，其性能与 NucliSens easyMAG 和 CAS-1200 系统相当，无论是定量还是定性。
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