W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.
Vaccine. 2011 Feb 11;29(8):1683-9. doi: 10.1016/j.vaccine.2010.12.040. Epub 2011 Jan 1.
Adenovirus particles can be engineered to display exogenous peptides on their surfaces by modification of viral capsid proteins, and particles that display pathogen-derived peptides can induce protective immunity. We constructed viable recombinant adenoviruses that display B-cell epitopes from the Plasmodium falciparum circumsporozoite protein (PfCSP) in the major adenovirus capsid protein, hexon. Recombinants induced high-titer antibodies against CSP when injected intraperitoneally into mice. Serum obtained from immunized mice recognized both recombinant PfCSP protein and P. falciparum sporozoites, and neutralized P. falciparum sporozoites in vitro. Replicating adenovirus vaccines have provided economical protection against adenovirus disease for over three decades. The recombinants described here may provide a path to an affordable malaria vaccine in the developing world.
腺病毒颗粒可以通过修饰病毒衣壳蛋白在其表面展示外源肽，并且展示病原体衍生肽的颗粒可以诱导保护性免疫。我们构建了活的重组腺病毒，在主要的腺病毒衣壳蛋白六邻体中展示疟原虫环子孢子蛋白（PfCSP）的 B 细胞表位。重组病毒经腹腔注射到小鼠体内后，可诱导针对 CSP 的高滴度抗体。从免疫小鼠获得的血清可识别重组 PfCSP 蛋白和疟原虫孢子，并在体外中和疟原虫孢子。复制型腺病毒疫苗已提供了超过三十年的针对腺病毒疾病的经济保护。这里描述的重组病毒可能为发展中国家提供一种负担得起的疟疾疫苗途径。
